Université de Pau et des Pays de l'Adour, E2S UPPA, CNRS IPREM, Environmental Microbiology and Chemistry, UMR 5254, 64000 Pau, France; TotalEnergies, OneTech, PERL ESD - Pôle D'Etudes et de Recherche de Lacq, Pôle Economique 2, BP 47 - RD 817, 64170 Lacq, France.
Politecnico di Milano, Department of Civil and Environmental Engineering (DICA), P.zza L. da Vinci, 32, 20133 Milan, Italy.
Bioresour Technol. 2024 Apr;397:130444. doi: 10.1016/j.biortech.2024.130444. Epub 2024 Feb 13.
The aim of this study was to acclimate anaerobic prokaryotes to saline microalgae biomass. Semi-continuous experiments were conducted using two 1.5 L mesophilic reactors for 10 weeks, (hydraulic retention time of 21 days). The first reactor was solely fed with sewage sludge (control), while the second received a mixture of sewage sludge and microalgal biomass (80/20 %w/w) cultivated at 70 g·L salinity. The in-reactor salinity reached after the acclimation phase was 14 g·L. Biomethane production was comparable between the control and acclimated reactors (205 ± 29 NmL·g). Salinity tolerance assessment of methanogenic archaea revealed that salinity causing 50% inhibition of methane production increased from 10 to 27 g·L after acclimation. Microbial diversity analyses revealed notable changes in methanogenic archaea populations during co-digestion of saline microalgae biomass, particularly methylotrophic (+27%) and acetotrophic (-26%) methanogens. This study has highlighted the possibility of treating efficiently saline microalgae in co-digestion with sewage sludge in future industrial biogas plants.
本研究旨在使厌氧原核生物适应盐度微藻生物质。使用两个 1.5 L 中温反应器进行了为期 10 周的半连续实验（水力停留时间为 21 天）。第一个反应器仅用污水污泥（对照）进料，而第二个反应器接收在 70 g·L 盐度下培养的污水污泥和微藻生物质（80/20 %w/w）的混合物。适应阶段后，反应器内的盐度达到 14 g·L。对照和驯化反应器中的生物甲烷产量相当（205 ± 29 NmL·g）。对产甲烷古菌的耐盐性评估表明，适应后导致甲烷产量抑制 50%的盐度从 10 增加到 27 g·L。微生物多样性分析表明，在与盐度微藻生物质共消化过程中，产甲烷古菌种群发生了显著变化，特别是甲基营养型（+27%）和乙酸营养型（-26%）产甲烷菌。本研究强调了在未来工业沼气厂中，用污水污泥共消化处理盐度微藻的可能性。
