转铁蛋白在人淋巴母细胞体外摄取金属中的作用。

Role of transferrin in metal uptake by human lymphoblasts in vitro.

作者信息

Planas-Bohne F, Taylor D M, Duffield J R

出版信息

Cell Biochem Funct. 1985 Jul;3(3):217-22. doi: 10.1002/cbf.290030309.

Abstract

The uptake and binding of 59Fe, 67Ga and 239Pu complexed with citrate of transferrin (Tf) and of 125I-labelled Fe-Tf by human lymphoblasts (WI-L2 cells) have been studied. Uptake kinetics of 59Fe-Tf and [125I]-Tf point to internalization by receptor mediated endocytosis. 67Ga binding and uptake is always less. This may be explained by a lower affinity of Ga-complexes for the cell surface. Factors which influence Fe uptake have a similar effect on Ga. 239Pu uptake and binding, however, are different, especially in that Tf does not stimulate 239Pu uptake and may actually decrease it.

摘要

已对人淋巴母细胞（WI-L2细胞）对与转铁蛋白（Tf）柠檬酸盐络合的59Fe、67Ga和239Pu以及125I标记的铁转铁蛋白（Fe-Tf）的摄取和结合进行了研究。59Fe-Tf和[125I]-Tf的摄取动力学表明其通过受体介导的内吞作用内化。67Ga的结合和摄取量始终较少。这可能是由于Ga络合物对细胞表面的亲和力较低。影响铁摄取的因素对Ga有类似影响。然而，239Pu的摄取和结合有所不同，特别是转铁蛋白不会刺激239Pu的摄取，实际上可能会降低其摄取量。