Banner B F, Gould V E, Radosevich J A, Ma Y, Lee I, Rosen S T
Diagn Cytopathol. 1985 Oct-Dec;1(4):300-7. doi: 10.1002/dc.2840010408.
Thirty-five pulmonary carcinomas were studied retrospectively with monoclonal antibody (MCA) 44-3A6 raised against a human adenocarcinoma cell line. The antibody was applied to cytologic smears of bronchial brushings originally stained with the Papanicolau method, and to conventional tissue sections. Ten of 12 adenocarcinomas (ADC) immunostained strongly in sections and smears, as did five of seven large-cell "undifferentiated" carcinomas (LCUC). Eight neuroendocrine carcinomas (NEC) and eight squamous-cell carcinomas (SCC) were negative, except for rare weakly positive foci. We conclude that MCA 44-3A6 can be effectively applied on cytologic smears, and that it could be valuable in the precise classification of pulmonary carcinomas. The immunoreactivity of the ADC and SCC was predictable. Positive immunostaining in some LCUC confirms that these constitute a heterogeneus tumor class that includes cases that are phenotypically ADC despite the absence of obvious glands. Occasional immunostaining in NEC suggests focal exocrine differentiation as previously noted by electron microscopy.
采用针对人腺癌细胞系产生的单克隆抗体（MCA）44 - 3A6对35例肺癌进行回顾性研究。该抗体应用于最初用巴氏染色法染色的支气管刷检细胞学涂片以及常规组织切片。12例腺癌（ADC）中有10例在切片和涂片中免疫染色强烈，7例大细胞“未分化”癌（LCUC）中有5例也是如此。8例神经内分泌癌（NEC）和8例鳞状细胞癌（SCC）呈阴性，仅见罕见的弱阳性灶。我们得出结论，MCA 44 - 3A6可有效地应用于细胞学涂片，并且在肺癌的精确分类中可能具有重要价值。ADC和SCC的免疫反应性是可预测的。一些LCUC中的阳性免疫染色证实这些构成了一类异质性肿瘤，其中包括一些尽管没有明显腺体但表型为ADC的病例。NEC中偶尔出现的免疫染色提示局灶性外分泌分化，正如先前电子显微镜所观察到的那样。
