Maayan C, Zajicek G, Rosenmann E
Int J Biomed Comput. 1979 Jan;10(1):23-8. doi: 10.1016/0020-7101(79)90039-4.
Human renal histological sections have been photographed on a black and white transparency and digitized. The digitizing camera, an image dissector, converts a 2 cm2 picture into 400 x 400 numbers each representing the grey level value of sampled point. The grey levels are represented in the computer by number ranging between 0-255. In this scale 'O' stands for black and 255, for white. The program provides 2 grey level thresholds, which outline a window through which the picture is scanned. Only structures, whose grey level value lies in the window range, are taken into consideration. The thresholds are set during digitization from the console switches. Image analysis is performed on-line in real time.
已对人类肾脏组织切片进行了黑白透明片拍摄并数字化处理。数字化相机(一种图像析象管)将一张2平方厘米的图片转换为400×400个数字,每个数字代表采样点的灰度值。灰度值在计算机中由0至255之间的数字表示。在此比例中,“0”代表黑色,“255”代表白色。该程序提供2个灰度阈值,勾勒出一个用于扫描图片的窗口。只有灰度值落在窗口范围内的结构才会被考虑。这些阈值在数字化过程中通过控制台开关进行设置。图像分析是实时在线进行的。