McDonagh P F, Cohen D M, Suaudeau J, Laks H
Microcirc Endothelium Lymphatics. 1985 Feb;2(1):67-84.
To determine the effects of ischemia and reperfusion on myocardial perfused capillary density, rat hearts were excised, kept ischemic for thirty minutes then reperfused with blood from a support animal. Control hearts were isolated and perfused for either 10 or 60 minutes before injection of a vascular marker (Monastral Blue-blood mixture, 30 sec at 100 mmHg). The ischemic hearts were perfused with Blue after 10 minutes of reperfusion. The perfused capillary density (theta) and capillary/fiber ratio (C/F) were measured from frozen transverse sections of the left ventricle. For 10- and 60-minute control hearts, the epicardial theta values were 2324 +/- 476 caps/mm2 and 2378 +/- 330 SD. The endo/epi theta ratio was not significantly less than unity for either control group. For the ischemic group, theta was significantly decreased both in the epicardium (707 +/- 515 caps/mm2) and endocardium (130 +/- 30 caps/mm2) (p less than 0.05). The endo/epi theta ratio was significantly less than unity for the ischemic group (P less than 0.05). The C/F ratio analysis yielded the same results. The decrease in both theta and CF ratio indicate that 30 minutes of ischemia followed by 10 minutes of reperfusion caused a marked "No-reflow" phenomena in isolated, supported hearts. The No-reflow was most pronounced in the endocardium.
为了确定缺血和再灌注对心肌灌注毛细血管密度的影响，切除大鼠心脏，使其缺血30分钟，然后用来自供体动物的血液进行再灌注。对照心脏分离后灌注10或60分钟，然后注射血管标记物（单星蓝 - 血液混合物，100 mmHg下30秒）。缺血心脏在再灌注10分钟后用蓝色染料灌注。从左心室的冷冻横切片测量灌注毛细血管密度（θ）和毛细血管/纤维比率（C/F）。对于10分钟和60分钟的对照心脏，心外膜θ值分别为2324±476个/mm²和2378±330个/mm²标准差。两个对照组的心内膜/心外膜θ比率均无显著低于1。对于缺血组，心外膜（707±515个/mm²）和心内膜（130±30个/mm²）的θ均显著降低（p<0.05）。缺血组的心内膜/心外膜θ比率显著低于1（P<0.05）。C/F比率分析得出相同结果。θ和C/F比率的降低表明，30分钟缺血后再灌注10分钟会在离体的、有支持的心脏中引起明显的“无复流”现象。无复流现象在心内膜最为明显。
