Adams G E, Posener M L, Bisby R H, Cundall R B, Key J R
Int J Radiat Biol Relat Stud Phys Chem Med. 1979 Jun;35(6):497-507. doi: 10.1080/09553007914550611.
The reactions of free radicals produced by ionizing radiation with pepsin have been studied by steady-state inactivation measurements and by pulse radiolysis. In de-aerated solutions thehydroxyl radical has been found to be the most efficient of the primary free radicals generated from water in causing inactivation. The reactions of the more selective oxidizing inorganic radical anions Br2-. and (SCN)2-., with pepsin have also beenexamined. In the case of the thiocyanate radical anion (SCN)2-., the inactivation efficiency is found to depend on SCN- concentration, an effect shown to arise from a reversible redox reaction involving the tryptophan and (SCN)2-. radicals. The results demonstrate that tryptophan residue plays an essential role in the enzyme activity of pepsin.
通过稳态失活测量和脉冲辐解研究了电离辐射产生的自由基与胃蛋白酶的反应。在脱气溶液中，已发现羟基自由基是由水产生的主要自由基中导致失活最有效的自由基。还研究了更具选择性的氧化性无机自由基阴离子Br2-.和(SCN)2-.与胃蛋白酶的反应。在硫氰酸根自由基阴离子(SCN)2-.的情况下，发现失活效率取决于SCN-浓度，这种效应表明是由涉及色氨酸和(SCN)2-.自由基的可逆氧化还原反应引起的。结果表明，色氨酸残基在胃蛋白酶的酶活性中起重要作用。
