鲍文病中人乳头瘤病毒的检出率：与盆腔和指（趾）部受累及特定的 p53 免疫染色模式的相关性。

Human papillomavirus detection rates in Bowen disease: correlation with pelvic and digital region involvement and specific p53 immunostaining patterns.

机构信息

Departments of Dermatology and Pathology, Guro Hospital, Korea University College of Medicine, Seoul, Republic of Korea.

Department of Pathology, Guro Hospital, Korea University College of Medicine, Seoul, Republic of Korea.

出版信息

Clin Exp Dermatol. 2024 Jul 19;49(8):848-858. doi: 10.1093/ced/llae050.

DOI:10.1093/ced/llae050

PMID:38365318

Abstract

BACKGROUND

The relationship between human papillomavirus (HPV) and Bowen disease (BD) is not fully understood.

OBJECTIVES

To investigate the differences in HPV detection rates in BD samples across various body regions and analyse the expression patterns of p53, p16 and Ki-67 in relation to HPV presence.

METHODS

Tissue samples from patients diagnosed with BD, confirmed through histopathology, were retrospectively collected. Next-generation sequencing was used for HPV DNA detection. Immunohistochemistry (IHC) for p16, p53 and Ki-67 was performed.

RESULTS

Out of 109 patients with BD, 21 (19.3%) were HPV-positive. All identified types were α-HPVs, with HPV-16 being the most common. The HPV detection rate was significantly higher in the pelvic (9/13, 69%, P < 0.001) and digital (5/10, 50%, P = 0.02) areas compared with those in the other regions. HPV presence was significantly correlated with p53 negativity (P = 0.002), the p53 'non-overexpression' IHC pattern (P < 0.001) and p16-p53 immunostain pattern discordance (P < 0.001). Conversely, there was no notable association between HPV presence and p16 positivity, the p16 IHC pattern or Ki-67 expression.

CONCLUSIONS

Our findings suggest the oncogenic role of sexually transmitted and genito-digitally transmitted α-HPVs in the pathogenesis of BD in pelvic and digital regions.

摘要

背景

人乳头瘤病毒（HPV）与 Bowen 病（BD）之间的关系尚未完全阐明。

目的

研究不同身体部位 BD 样本中 HPV 检出率的差异，并分析 HPV 存在与 p53、p16 和 Ki-67 表达模式之间的关系。

方法

回顾性收集经组织病理学证实的 BD 患者的组织样本。采用下一代测序技术检测 HPV DNA。行 p16、p53 和 Ki-67 的免疫组织化学（IHC）检测。

结果

在 109 例 BD 患者中，21 例（19.3%）HPV 阳性。所有鉴定的类型均为 α-HPV，其中 HPV-16 最为常见。与其他部位相比，盆腔（9/13，69%，P < 0.001）和指部（5/10，50%，P = 0.02）的 HPV 检出率显著更高。HPV 阳性与 p53 阴性（P = 0.002）、p53“非过表达”IHC 模式（P < 0.001）和 p16-p53 免疫染色模式不匹配（P < 0.001）显著相关。相反，HPV 阳性与 p16 阳性、p16 IHC 模式或 Ki-67 表达之间无明显相关性。