Mitsuishi T, Sata T, Matsukura T, Iwasaki T, Kawashima M
Department of Dermatology, Tokyo Women's Medical College, Shinjuku-ku, Japan.
Cancer. 1997 May 15;79(10):1911-7. doi: 10.1002/(sici)1097-0142(19970515)79:10<1911::aid-cncr11>3.0.co;2-y.
Bowen's disease (BD) of the genital skin region is generally associated with human papillomavirus (HPV) infection. Various molecular analyses have identified mainly HPV-16 in the lesions. However, the HPV genotypes associated with BD of the hands have not yet been characterized.
The skin specimens of 12 patients with BD of the hands were investigated clinicopathologically and immunohistochemically, and the total DNAs extracted from the skin were analyzed for the presence of HPV DNA using Southern blot hybridization and polymerase chain reaction (PCR) in combination with restriction fragment length polymorphisms (RFLP) and direct sequencing analysis of the amplified products. In addition, the histologic localization of HPV DNA was examined by in situ hybridization in paraffin embedded sections of HPV positive patients.
In 8 of 12 BD lesions (66.7%), HPV types (HPV-16, -31, -54, -58, -61, -62, and -73) were detected by Southern blot hybridization and/or PCR with RFLP and direct sequencing analysis. In 6 of 7 HPV positive lesions examined (85.7%), the viral genomes were identified by in situ hybridization in the nuclei of keratinocytes in the upper stratum malpighii and/or stratum corneum.
These results demonstrate that BD of the hands is frequently associated with HPV infection. The seven HPV genotypes are known as mucosal (genital) HPVs and to the authors' knowledge, this is the first time HPV-31, -54, -58, -61, -62, and -73 have been identified in BD lesions. These findings strongly suggest that HPVs related to mucosal lesions play an important role in the development of BD of the hands.
生殖器皮肤区域的鲍温病（BD）通常与人乳头瘤病毒（HPV）感染相关。各种分子分析已在病变中主要鉴定出HPV - 16。然而，与手部BD相关的HPV基因型尚未得到明确特征描述。
对12例手部BD患者的皮肤标本进行临床病理和免疫组织化学研究，并使用Southern印迹杂交和聚合酶链反应（PCR）结合限制性片段长度多态性（RFLP）以及对扩增产物进行直接测序分析，检测从皮肤中提取的总DNA中HPV DNA的存在情况。此外，通过原位杂交在HPV阳性患者的石蜡包埋切片中检查HPV DNA的组织学定位。
在12个BD病变中的8个（66.7%）中，通过Southern印迹杂交和/或PCR结合RFLP及直接测序分析检测到HPV类型（HPV - 16、-31、-54、-58、-61、-62和-73）。在7个检测的HPV阳性病变中的6个（85.7%）中，通过原位杂交在上皮基底上层和/或角质层的角质形成细胞核中鉴定出病毒基因组。
这些结果表明手部BD常与HPV感染相关。这七种HPV基因型被认为是黏膜（生殖器）型HPV，据作者所知，这是首次在BD病变中鉴定出HPV - 31、-54、-58、-61、-62和-73。这些发现强烈表明与黏膜病变相关的HPV在手部BD的发生中起重要作用。
