利用声力学光谱探测人类免疫细胞力学性质

Probing human immune cell mechanics using acoustic force spectroscopy.

机构信息

Medical Systems Biophysics and Bioengineering, Leiden Academic Centre for Drug Research, Faculty of Mathematics and Natural Sciences, Leiden University, Einsteinweg 55, 2333CC Leiden, the Netherlands; Laboratory for Interdisciplinary Medical Innovations, Centre for Interdisciplinary Genome Research, Leiden University, Einsteinweg 55, 2333CC Leiden, the Netherlands.

出版信息

STAR Protoc. 2024 Mar 15;5(1):102861. doi: 10.1016/j.xpro.2024.102861. Epub 2024 Feb 16.

DOI:10.1016/j.xpro.2024.102861

PMID:38367234

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10879795/

Abstract

Immune cells continuously adapt their mechanical properties for proper circulation and elicitation of immune responses. Here, we provide a step-by-step protocol for probing the single-cell mechanical properties of primary human monocytes using acoustic force spectroscopy (AFS). We describe steps for the calibration of the AFS chips, the isolation of monocytes from buffy coats, and the probing of monocyte mechanics using AFS. We then detail the data analysis strategy. The protocol is useful for characterizing a wide range of immune cells under various conditions in physiology and pathology. For complete details on the use and execution of this protocol, please refer to Evers et al. and Evers et al..

摘要

免疫细胞不断调整其力学特性以实现适当的循环和免疫应答。在这里，我们提供了一个使用声镊力谱（AFS）探测原代人单核细胞单细胞力学特性的分步协议。我们描述了 AFS 芯片的校准、从血沉棕黄层中分离单核细胞以及使用 AFS 探测单核细胞力学的步骤。然后，我们详细介绍了数据分析策略。该方案可用于在生理和病理条件下对各种条件下的多种免疫细胞进行特征描述。有关此协议的使用和执行的完整详细信息，请参阅 Evers 等人和 Evers 等人的研究。