Department of Chemistry "Ugo Schiff" and CSGI, University of Florence, Sesto Fiorentino, Firenze 50019, Italy.
Department of Chemistry and Pharmacy, University of Sassari, Sassari 07100, Italy.
Colloids Surf B Biointerfaces. 2024 Apr;236:113789. doi: 10.1016/j.colsurfb.2024.113789. Epub 2024 Feb 6.
The effects of a range of electrolytes on the hydrolysis of urea by the enzyme urease is explored. The autocatalytic behavior of urease in unbuffered solutions and its pH clock reactions are studied. The concentration dependence of the experimental variables is analyzed in terms of specific ion-enzyme interactions and hydration. The results offer insights into the molecular mechanisms of the enzyme, and on the nature of its interactions with the electrolytes. We found that urease can tolerate mild electrolytes in its environment, while it is strongly inhibited by both strong kosmotropic and strong chaotropic anions. This study may cast light on an alternative therapy for Helicobacter pylori infections and contribute to the design of innovative materials and provide new approaches for the modulation of the enzymatic activity.
研究了一系列电解质对酶脲酶水解尿素的影响。研究了未缓冲溶液中脲酶的自动催化行为及其 pH 钟反应。根据特定的离子-酶相互作用和水合作用分析了实验变量的浓度依赖性。研究结果深入了解了酶的分子机制及其与电解质相互作用的本质。我们发现，脲酶可以在其环境中耐受温和的电解质，但它会被强亲脂性和强亲水性阴离子强烈抑制。这项研究可能为幽门螺杆菌感染的替代疗法提供启示，并有助于设计创新材料和为调节酶活性提供新方法。
