溶血磷脂酸（LPA）受体介导的信号传导在缺氧条件下胰腺癌细胞中内皮细胞调节的细胞功能中的作用。
Roles of lysophosphatidic acid (LPA) receptor-mediated signaling in cellular functions modulated by endothelial cells in pancreatic cancer cells under hypoxic conditions.
作者信息
Takai Miwa, Yashiro Narumi, Hara Koki, Amano Yuka, Yamamoto Mao, Tsujiuchi Toshifumi
机构信息
Division of Molecular Oncology, Department of Life Science, Faculty of Science and Engineering, Kindai University, 3-4-1, Kowakae, Higashiosaka, Osaka 577-8502, Japan.
Division of Molecular Oncology, Department of Life Science, Faculty of Science and Engineering, Kindai University, 3-4-1, Kowakae, Higashiosaka, Osaka 577-8502, Japan.
出版信息
Pathol Res Pract. 2024 Mar;255:155192. doi: 10.1016/j.prp.2024.155192. Epub 2024 Feb 6.
BACKGROUND
In the tumor environment, malignant characteristics of cancer cells are promoted by stromal cells under hypoxia. It is unknown whether lysophosphatidic acid (LPA) receptor-mediated signaling is involved in the regulation of cellular functions by endothelial cells in pancreatic cancer cells under hypoxic conditions.
METHODS
Pancreatic cancer (PANC-1) cells were co-cultured with endothelial (F2) cells and F2 cell supernatants at 21% and 1% O. The Cell Culture Insert was used to assess the cell motile activity. The cell growth and viability to cisplatin (CDDP) were measured, using the Cell Counting Kit-8.
RESULTS
LPA receptor expression levels were changed in PANC-1 cells co-cultured with F2 cells at 21% and 1% O. The cell motile activities of PANC-1 cells co-cultured with F2 cells at 21% and 1% O were markedly elevated, compared with PANC-1 cells alone. The cell viabilities to CDDP of PANC-1 cells co-cultured with F2 cell supernatants at 21% and 1% O were regulated by the activation of LPA receptors.
CONCLUSION
These results suggest that LPA receptor-mediated signaling plays an important role in the modulation of pancreatic cancer cell functions by endothelial cells under hypoxic conditions.
背景
在肿瘤环境中，缺氧条件下基质细胞可促进癌细胞的恶性特征。尚不清楚溶血磷脂酸（LPA）受体介导的信号传导是否参与缺氧条件下内皮细胞对胰腺癌细胞细胞功能的调节。
方法
将胰腺癌细胞（PANC-1）与内皮细胞（F2）以及F2细胞上清液在21%和1%氧气浓度下共培养。使用细胞培养插入物评估细胞运动活性。使用细胞计数试剂盒-8测量细胞生长以及对顺铂（CDDP）的活力。
结果
在21%和1%氧气浓度下与F2细胞共培养的PANC-1细胞中，LPA受体表达水平发生变化。与单独的PANC-1细胞相比，在21%和1%氧气浓度下与F2细胞共培养的PANC-1细胞的运动活性显著提高。在21%和1%氧气浓度下与F2细胞上清液共培养的PANC-1细胞对CDDP的细胞活力受LPA受体激活的调节。
结论
这些结果表明，LPA受体介导的信号传导在缺氧条件下内皮细胞对胰腺癌细胞功能的调节中起重要作用。
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