Emig Alyssa A, Hansen Megan, Grimm Sandra, Coarfa Cristian, Lord Nathan D, Williams Margot Kossmann
Center for Precision Environmental Health and Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX.
Dan L Duncan Comprehensive Cancer Center, Baylor College of Medicine, Houston, TX.
bioRxiv. 2024 Feb 7:2024.02.06.579243. doi: 10.1101/2024.02.06.579243.
Anteroposterior (AP) elongation of the vertebrate body plan is driven by convergence and extension (C&E) gastrulation movements in both the mesoderm and neuroectoderm, but how or whether molecular regulation of C&E differs between tissues remains an open question. Using a zebrafish explant model of AP axis extension, we show that C&E of the neuroectoderm and mesoderm can be uncoupled , and that morphogenesis of individual tissues results from distinct morphogen signaling dynamics. Using precise temporal manipulation of BMP and Nodal signaling, we identify a critical developmental window during which high or low BMP/Nodal ratios induce neuroectoderm- or mesoderm-driven C&E, respectively. Increased BMP activity similarly enhances C&E specifically in the ectoderm of intact zebrafish gastrulae, highlighting the relevance of our findings. Together, these results demonstrate that temporal dynamics of BMP and Nodal morphogen signaling activate distinct morphogenetic programs governing C&E gastrulation movements within individual tissues.
脊椎动物身体结构的前后（AP）伸长是由中胚层和神经外胚层中的汇聚延伸（C&E）原肠胚形成运动驱动的，但C&E在不同组织间的分子调控方式或是否存在差异仍是一个悬而未决的问题。利用斑马鱼AP轴延伸的外植体模型，我们发现神经外胚层和中胚层的C&E可以解偶联，并且单个组织的形态发生源于不同的形态发生素信号动态变化。通过对BMP和Nodal信号进行精确的时间操纵，我们确定了一个关键的发育窗口，在此期间，高或低的BMP/Nodal比值分别诱导神经外胚层或中胚层驱动的C&E。增加BMP活性同样会特异性增强完整斑马鱼原肠胚外胚层中的C&E，突出了我们研究结果的相关性。总之，这些结果表明，BMP和Nodal形态发生素信号的时间动态激活了不同的形态发生程序，这些程序控制着单个组织内的C&E原肠胚形成运动。
