Department of Cardiovascular Medicine, The First People´s Hospital of Liping District, Hangzhou, Zhejiang, China.
Cell Mol Biol (Noisy-le-grand). 2024 Jan 31;70(1):119-127. doi: 10.14715/cmb/2024.70.1.16.
We aimed to study the association between the non-coding region of the lncRNA MALAT1 gene, the non-coding region rs664589 C>G variant, and the risk of acute myocardial infarction (AMI) in the Chinese Han population. 165 NSTEMI and 135 STEMI patients were enrolled in the study. An additional 150 healthy individuals were enrolled as the controls. All subjects were analyzed for the MALAT1 rs664589 locus genotype. The receiver operating curve (ROC) was used to determine the effect of MALAT1 rs664589 single nucleotide polymorphism (SNP) on the diagnosis of AMI by plasma lncRNA MALAT1. The MALAT1 rs664589 site G allele carrier was 1.39 times more likely to have NSTEMI than the C allele carrier (95% CI: 1.16-1.61, P = 0.001) and 1.59 times more likely to have STEMI than the C allele carrier (95% CI: 1.31-1.85, P < 0.001). The MALAT1 rs664589 site C>G mutation resulted in an increase in the area under the ROC curve (AUC) of the plasma lncRNA MALAT1 level for the diagnosis of AMI. The plasma lncRNA MALAT1 levels in AMI patients were negatively correlated with hsa-miR-1972, hsa-miR-194-5p, hsa-miR-4717-5p, hsa-miR-6735-3p, and hsa-miR-3677-5p (r = -0.81, -0.75, -0.66, -0.71, and -0.88). The C>G mutation of MAL6641 rs664589 causes an increased risk of AMI in the Chinese Han population. The SNP at this site affects the value of plasma lncRNA MALAT1 in the diagnosis of AMI. The specific mechanism may indicate that the C>G mutation of the MALAT1 rs664589 changes the regulation of miRNAs expression by lncRNA MALAT1.
我们旨在研究长链非编码 RNA MALAT1 基因的非编码区、非编码区 rs664589 C>G 变体与中国汉族人群急性心肌梗死（AMI）风险之间的关系。研究纳入了 165 例 NSTEMI 患者和 135 例 STEMI 患者。另外还招募了 150 名健康个体作为对照组。对所有受试者进行 MALAT1 rs664589 基因座基因型分析。采用受试者工作特征曲线（ROC）确定 MALAT1 rs664589 单核苷酸多态性（SNP）对血浆 lncRNA MALAT1 诊断 AMI 的影响。MALAT1 rs664589 位点 G 等位基因携带者发生 NSTEMI 的可能性比 C 等位基因携带者高 1.39 倍（95%CI：1.16-1.61，P=0.001），发生 STEMI 的可能性比 C 等位基因携带者高 1.59 倍（95%CI：1.31-1.85，P<0.001）。MALAT1 rs664589 位点 C>G 突变导致血浆 lncRNA MALAT1 水平诊断 AMI 的 ROC 曲线下面积（AUC）增加。AMI 患者的血浆 lncRNA MALAT1 水平与 hsa-miR-1972、hsa-miR-194-5p、hsa-miR-4717-5p、hsa-miR-6735-3p 和 hsa-miR-3677-5p 呈负相关（r=-0.81、-0.75、-0.66、-0.71 和-0.88）。MAL6641 rs664589 的 C>G 突变使中国汉族人群发生 AMI 的风险增加。该位点的 SNP 影响了血浆 lncRNA MALAT1 诊断 AMI 的价值。具体机制可能表明 MALAT1 rs664589 的 C>G 突变改变了 lncRNA MALAT1 对 miRNA 表达的调控。
