Laboratory of Applied Microbiology, Faculty of Microbiology, Ferhat Abbas University, Setif, Algeria.
Higher National School of Biotechnology, Taoufik Khaznadar University, Constantine, Algeria.
Cell Mol Biol (Noisy-le-grand). 2024 Jan 31;70(1):67-79. doi: 10.14715/cmb/2024.70.1.10.
Bacillus sp. RTS11, a xylanolytic strain, was isolated from the Algerian desert rocks. Genetic analysis revealed a remarkable 98.69% similarity to Bacillus pumilus. We harnessed optimization techniques, including Plackett-Burman screening and Box-Behnken optimization design, to amplify xylanase production and activity. The outcome of these efforts was an optimized medium that yielded an impressive xylanase production titer of 448.89 U, a threefold increase compared to the non-optimized medium (146 U). The Purification of xylanase was achieved through the three-phase partitioning technique, employing t-butanol and various chromatographic methods. Notably, anion exchange chromatography led to isolating a highly pure enzyme with a molecular weight of 60 kDa. The xylanase exhibited its peak activity at a temperature of 60°C and a pH of 9.0. When applied to pulp pretreatment, 20 U/g of xylanase demonstrated a substantial increase in the release of phenolic and chromophore compounds while reducing sugar content in the pulp. Furthermore, this versatile xylanase shows its ability to efficiently hydrolyze a variety of agro-industrial residues, including wheat bran, corn and grape waste, wheat straw, and sugarcane bagasse. These findings underscore the significant potential of this xylanase enzyme in biobleaching processes and the utilization of agro-industrial waste, opening up exciting avenues for sustainable and environmentally friendly industrial applications.
从阿尔及利亚沙漠岩石中分离到一株木聚糖酶产生菌芽孢杆菌 sp. RTS11，其与解淀粉芽孢杆菌的基因序列相似性高达 98.69%。利用 Plackett-Burman 筛选和 Box-Behnken 优化设计技术对菌株进行优化，使木聚糖酶的产量和活力得到显著提高。优化后的培养基使木聚糖酶的产量达到 448.89 U，比未优化的培养基（146 U）提高了 3 倍。通过三相分配技术，采用叔丁醇和各种色谱方法对木聚糖酶进行纯化。阴离子交换色谱分离得到一种具有 60 kDa 分子量的高纯度酶。木聚糖酶的最适反应温度和 pH 值分别为 60°C 和 9.0。在纸浆预处理中，20 U/g 的木聚糖酶可显著提高纸浆中酚类和发色团化合物的释放量，同时降低还原糖含量。此外，这种多功能木聚糖酶还能够有效地水解多种农业工业废弃物，包括麦麸、玉米和葡萄废物、麦草和甘蔗渣。这些发现突显了该木聚糖酶在生物漂白过程和农业工业废物利用中的巨大潜力，为可持续和环保的工业应用开辟了令人兴奋的途径。
