Wolin S L, Kucherlapati R S
J Cell Biol. 1979 Jul;82(1):76-85. doi: 10.1083/jcb.82.1.76.
Microtubules play an important role in several cellular functions including cellular architecture and chromosome movement in cell division. Tubulin which polymerizes to form mictobules can be purified to homogeneity and used to raised antisera. Antisera prepared against porcine or chicken tubulin reacts well with mammalian tubulin. We have examined normal and transformed cells of mouse and human origin for microtubules by indirect immunofluorescence methods. Extensive networks of microtubules (MN) are easily detectable in normal and some transformed cells. The fixation procedure employed and the morphology and the cellular attachment properties seem to determine the ease of detection of MN in these cells. Cells derived from tumors and exhibiting several transformed phenotypes contained MN comparable to those of normal cells. Hybrids between transformed mouse cells and normal human cells were examined. They showed a variability in morphology, but all contained MN. These hybrids exhibited several transformed phenotypes. We conclude that in the cell lines we have examined there is no correlation between the transformed phenotypes and the organization of tubulin.
微管在包括细胞结构和细胞分裂中的染色体移动等多种细胞功能中发挥着重要作用。聚合形成微管的微管蛋白可以被纯化至同质状态，并用于制备抗血清。针对猪或鸡微管蛋白制备的抗血清与哺乳动物微管蛋白反应良好。我们已经通过间接免疫荧光法检查了小鼠和人类来源的正常细胞和转化细胞中的微管。在正常细胞和一些转化细胞中很容易检测到广泛的微管网（MN）。所采用的固定程序以及细胞的形态和附着特性似乎决定了在这些细胞中检测MN的难易程度。源自肿瘤并表现出几种转化表型的细胞所含的MN与正常细胞相当。对转化的小鼠细胞和正常人类细胞之间的杂种进行了检查。它们在形态上表现出变异性，但都含有MN。这些杂种表现出几种转化表型。我们得出结论，在我们检查的细胞系中，转化表型与微管蛋白的组织之间没有相关性。
