Davidson R L, O'Malley K A, Wheeler T B
Somatic Cell Genet. 1976 May;2(3):271-80. doi: 10.1007/BF01538965.
The effects of polyethylene glycol (PEG) molecular weight and concentration on mammalian cell hybridization were studied. The peak hybridization-inducing activity with all grades of PEG from 400-6000 was found to occur in the concentration range of 50-55%. However, changes in concentration were seen to have different quantitative effects with different grades of PEG. For monolayer fusions, PEG 1000 at 50% seems to be the optimal combination of PEG molecular weight and concentration, in terms of both efficiency of hybridization and relative insensitivity to dilution effects.
研究了聚乙二醇（PEG）分子量和浓度对哺乳动物细胞杂交的影响。发现400 - 6000所有等级的PEG诱导杂交的活性峰值出现在50 - 55%的浓度范围内。然而，浓度变化对不同等级的PEG有不同的定量影响。对于单层融合，就杂交效率和对稀释效应相对不敏感而言，50%的PEG 1000似乎是PEG分子量和浓度的最佳组合。
