Khamies Maged, Hagar Mohamed, Kassem Taher S E, Moustafa Amira Hossam Eldin
Chemistry Department, Faculty of Science, Alexandria University, P.O. 426 Ibrahemia, Alexandria, 21321, Egypt.
Faculty of Advanced Basic Sciences, Alamein International University, Alamein City, Matrouh Governorate, Egypt.
Sci Rep. 2024 Feb 19;14(1):4064. doi: 10.1038/s41598-024-53865-9.
The vegetable oil degumming process plays a critical role in refining edible oil. Phospholipids (PL) removal from crude extracted soybean oil (SBO) by the enzymatic degumming process has been investigated in this work. Enzymatic degumming of extracted SBO with microbial phospholipase A1 PLA-1 Quara LowP and Lecitase Ultra enzymes have also been studied comparatively. The main novelty of our work is the use of the enzymatic degumming process on an industrial scale (600 tons a day). Many parameters have been discussed to understand in detail the factors affecting oil losses during the degumming process. The factors such as chemical conditioning (CC) by phosphoric acid 85%, the enzyme dosage mg/kg (feedstock dependent), the enzymatic degumming reaction time, and the characteristics of the plant-processed SBO have been discussed in detail. As a main point, the degummed oil with a phosphorus content of < 10 mg/kg increases yield. Quara LowP and Lecitase Ultra enzymes are not specific for certain phospholipids PL; however, the conversion rate depends on the SBO phospholipid composition. After 4 h, over 99% of Phospholipids were degraded to their lysophospholipid LPL (lysolecithin). The results showed a significant effect of operating parameters and characteristics of different origins of SBO, fatty acids FFA content, Phosphorus content and total divalent metals (Calcium Ca, Magnesium Mg and Iron Fe mg/kg) content on the oil loss. The benefit of using enzymatic degumming of vegetable oils rather than traditional chemical refining is that the enzymatic degumming process reduces total oil loss. This decrease is known as enzymatic yield. The enzymatic degumming also decreases wastewater and used chemicals and running costs; moreover, it enables physical refining by lowering the residue phosphorus to < 10 mg/kg.
植物油脱胶工艺在食用油精炼过程中起着关键作用。本研究对通过酶法脱胶工艺从粗提大豆油(SBO)中去除磷脂(PL)进行了探究。同时还对使用微生物磷脂酶A1(PLA - 1)Quara LowP和Lecitase Ultra酶对提取的SBO进行酶法脱胶进行了比较研究。我们工作的主要创新点在于酶法脱胶工艺的工业规模应用(每天600吨)。为详细了解脱胶过程中影响油脂损失的因素,讨论了许多参数。详细讨论了诸如用85%磷酸进行化学调质(CC)、酶用量mg/kg(取决于原料)、酶法脱胶反应时间以及工厂加工的SBO的特性等因素。重点是,磷含量<10 mg/kg的脱胶油可提高产量。Quara LowP和Lecitase Ultra酶对某些磷脂(PL)不具有特异性;然而,转化率取决于SBO的磷脂组成。4小时后,超过99%的磷脂被降解为溶血磷脂(LPL,溶血卵磷脂)。结果表明,操作参数以及不同产地SBO的特性、游离脂肪酸(FFA)含量、磷含量和总二价金属(钙Ca、镁Mg和铁Fe mg/kg)含量对油脂损失有显著影响。使用植物油酶法脱胶而非传统化学精炼的好处在于酶法脱胶工艺可减少总油脂损失。这种减少被称为酶法得率。酶法脱胶还可减少废水、化学品用量和运行成本;此外,通过将残留磷降低至<10 mg/kg,可实现物理精炼。