Mihai Mara Mădălina, Popa Mircea Ioan, Holban Alina Maria, Gheorghe-Barbu Irina, Popa Liliana Gabriela, Chifiriuc Mariana-Carmen, Giurcăneanu Călin, Bleotu Coralia, Cucu Corina Ioana, Lazăr Veronica
Department of Oncologic Dermatology-"Elias" University Emergency Hospital, "Carol Davila" University of Medicine and Pharmacy, Bucharest, Romania.
Department of Botany-Microbiology, Faculty of Biology, Research Institute of the University of Bucharest, University of Bucharest, Bucharest, Romania.
Front Microbiol. 2024 Feb 5;14:1326904. doi: 10.3389/fmicb.2023.1326904. eCollection 2023.
Chronic venous ulcers of the lower limbs develop in the context of advanced venous disease and have a significant impact on the patient's quality of life, being associated with depression and worrisome suicide rates, as well as with an economic burden caused by increased medical care costs and high epidemiological risks of healthcare associated infections and emergence of strains resistant to multiple classes of antibiotics and/ or antiseptics. Although numerous studies have investigated the composition of the chronic wounds microbiome, either by culture-dependent or independent methods, there are no data on the association between virulence and resistance profiles of strains isolated from venous ulcers and the clinical picture of this pathology. The elucidation of pathogenic mechanisms, at both phenotypic and molecular level, is crucial in the fight against these important human microbial agents, in order to develop novel biomarkers and discover new therapeutic targets.
In this study we aimed to characterize the phenotypic virulence profiles (including the ability to develop biofilms) of microorganisms isolated from chronic skin wounds and to correlate them with the clinical symptomatology. Considering the high incidence of infections in chronic ulcers, but also the ability of this species to develop multi-drug resistance, we performed an more in-depth study of the phenotypic and genotypic virulence profiles of methicillin-resistant .
The study revealed important differences regarding the clinical evolution and virulence profiles of microorganisms isolated from lower limb wounds, as well as between patients diagnosed with chronic venous ulcers and those with lesions of different etiology.
下肢慢性静脉溃疡在晚期静脉疾病的背景下发生，对患者的生活质量有重大影响，与抑郁症和令人担忧的自杀率相关，还与医疗护理成本增加、医疗保健相关感染的高流行病学风险以及多重耐药菌株和/或防腐剂的出现所造成的经济负担有关。尽管众多研究已通过培养依赖性或非依赖性方法调查了慢性伤口微生物群的组成，但关于从静脉溃疡分离出的菌株的毒力和耐药性特征与这种病理的临床症状之间的关联尚无数据。在表型和分子水平上阐明致病机制对于对抗这些重要的人类微生物病原体至关重要，以便开发新的生物标志物并发现新的治疗靶点。
在本研究中，我们旨在表征从慢性皮肤伤口分离出的微生物的表型毒力特征（包括形成生物膜的能力），并将它们与临床症状相关联。考虑到慢性溃疡中感染的高发生率，以及该菌种产生多重耐药性的能力，我们对耐甲氧西林菌的表型和基因型毒力特征进行了更深入的研究。
该研究揭示了从下肢伤口分离出的微生物在临床演变和毒力特征方面的重要差异，以及在诊断为慢性静脉溃疡的患者与病因不同的病变患者之间的差异。
