State Key Laboratory of Environmental Criteria and Risk Assessment, National Engineering Laboratory for Lake Pollution Control and Ecological Restoration, National Environmental Protection Dongting Lake Scientific Observation and Research Station, State Environmental Protection Key Laboratory for Lake Pollution Control, Chinese Research Academy of Environmental Sciences, Beijing 100012, China.
Global Centre for Environmental Remediation, University of Newcastle, Callaghan, NSW 2308, Australia.
Environ Sci Process Impacts. 2024 Apr 24;26(4):700-709. doi: 10.1039/d3em00413a.
Perfluorooctanoic acid (PFOA) is an emerging persistent organic pollutant. Exposure to PFOA was observed to have a correlation with the expression levels of phospholipids. However, there are currently no studies that directly visualize the effects of PFOA on phospholipids. To this end, matrix-assisted laser desorption/ionization time of flight imaging mass spectrometry (MALDI-TOF-IMS) was used to visualize changes in phospholipids in the different tissues of zebrafish following exposure to PFOA. This study found that the major perturbed phospholipids were phosphatidylcholine (PC), diacylglycerol (DG), phosphatidic acid (PA), phosphatidylglycerol (PG), sphingomyelin (SM), and triacylglycerol (TG). These perturbed phospholipids caused by PFOA were reversible in some tissues (liver, gill, and brain) and irreversible in others (such as the highly exposed intestine). Moreover, the spatial distribution of perturbed phospholipids was mainly located around the edge or center of the tissues, implying that these tissue regions need special attention. This study provides novel insight into the biological toxicity and toxicity mechanisms induced by emerging environmental pollutants.
全氟辛酸（PFOA）是一种新兴的持久性有机污染物。有研究表明，接触 PFOA 与磷脂的表达水平有关。然而，目前尚无研究直接观察到 PFOA 对磷脂的影响。为此，本研究采用基质辅助激光解吸/电离飞行时间成像质谱（MALDI-TOF-IMS）来可视化斑马鱼在暴露于 PFOA 后不同组织中磷脂的变化。研究发现，受干扰的主要磷脂是磷脂酰胆碱（PC）、二酰基甘油（DG）、磷脂酸（PA）、磷脂酰甘油（PG）、鞘磷脂（SM）和三酰基甘油（TG）。这些由 PFOA 引起的受干扰磷脂在一些组织（肝脏、鳃和大脑）中是可逆的，而在其他组织（如高度暴露的肠道）中是不可逆的。此外，受干扰磷脂的空间分布主要位于组织的边缘或中心附近，这意味着这些组织区域需要特别关注。本研究为新兴环境污染物引起的生物毒性和毒性机制提供了新的见解。
