Department of Clinical Laboratory, Beijing Shijitan Hospital, Capital Medical University, Beijing, China.
Beijing Key Laboratory of Urinary Cellular Molecular Diagnostics, Beijing, China.
Braz J Med Biol Res. 2024 Feb 19;57:e13192. doi: 10.1590/1414-431X2023e13192. eCollection 2024.
The aim of this study was to explore the association between differential percentages of dendritic cell (DC) subsets in peripheral blood and malignancy (grade and lymph node metastasis) of peritoneal adenocarcinoma patients and the frequencies of dendritic cell subsets in the normal controls. The peripheral blood of 30 patients with peritoneal adenocarcinoma and 12 healthy controls were collected for multicolor flow cytometry analysis. Peritoneal adenocarcinoma patients were grouped according to the malignant degree (grade and lymph node metastasis). Percentages of myeloid DCs (mDCs) and its subsets MDC1 and MDC2 in DCs were lower in peripheral blood of patients with peritoneal adenocarcinoma than in normal controls. The percentages of plasmacytoid dendritic cells (pDCs) and CD16+mDCs in DCs were higher than in normal controls. Compared with poor differentiation grade, patients with well/moderate differentiation grade had an increased percentage of CD16+mDCs. Contrary to CD16+mDCs, the percentage of MDC1 was lower in the well/moderate differentiation grade group. In patients with no lymph node metastasis, pDCs and CD16+mDCs levels were higher compared with patients with lymph node metastasis. mDCs and MDC1 levels had opposite results. pDCs were positively correlated with CD16+mDCs in peripheral blood of peritoneal patients, as was mDCs and MDC1. CD16+mDCs were negatively correlated with MDC1. The percentages of pDCs and CD16+mDCs in DCs were positively correlated with CD3+CD8+T cells, and pDCs also positively correlated with CD8+PD-1+T cells. Our results revealed that DCs subsets correlated with peritoneal adenocarcinoma malignancy. Dendritic cells play an independent role in the immune function of peritoneal adenocarcinoma.
本研究旨在探讨外周血树突状细胞（DC）亚群的差异百分比与腹膜腺癌患者恶性程度（分级和淋巴结转移）的关系，并分析其与正常对照组之间的差异。采集 30 例腹膜腺癌患者和 12 例健康对照者的外周血，采用多色流式细胞术分析。根据恶性程度（分级和淋巴结转移）对腹膜腺癌患者进行分组。结果显示，与健康对照组相比，腹膜腺癌患者外周血中髓样 DC（mDC）及其亚群 MDC1 和 MDC2 的百分比较低，而浆细胞样 DC（pDC）和 CD16+mDC 的百分比较高。与低分化组相比，中高分化组 CD16+mDC 的百分比增加。与 CD16+mDC 相反，MDC1 的百分比在中高分化组中较低。在无淋巴结转移的患者中，pDC 和 CD16+mDC 的水平高于有淋巴结转移的患者。mDC 和 MDC1 的水平则相反。pDC 与外周血中 CD16+mDC 呈正相关，mDC 与 MDC1 也呈正相关。CD16+mDC 与 MDC1 呈负相关。pDCs 和 CD16+mDCs 在 DC 中的百分比与 CD3+CD8+T 细胞呈正相关，pDCs 与 CD8+PD-1+T 细胞也呈正相关。我们的研究结果表明，DC 亚群与腹膜腺癌的恶性程度相关。树突状细胞在腹膜腺癌的免疫功能中发挥独立作用。
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