辣椒提取物、辣椒素和二氢辣椒素作为靶向拓扑异构酶的潜在抗癌剂。

Chili pepper extracts, capsaicin, and dihydrocapsaicin as potential anticancer agents targeting topoisomerases.

机构信息

Department of Medical Biology, Faculty of Medicine, Pavol Jozef Šafárik University in Košice, Trieda SNP 1, 040 11, Košice, Slovakia.

Department of Chemistry, Biochemistry and Biophysics, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81, Košice, Slovakia.

出版信息

BMC Complement Med Ther. 2024 Feb 21;24(1):96. doi: 10.1186/s12906-024-04394-5.

DOI:10.1186/s12906-024-04394-5

PMID:38383414

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10880293/

Abstract

DNA topoisomerases regulate conformational changes in DNA topology during normal cell growth, such as replication, transcription, recombination, and repair, and may be targeted for anticancer drugs. A DNA topology assay was used to investigate DNA-damaging/protective activities of extracts from Habanero Red (HR), Habanero Maya Red (HMR), Trinidad Moruga Scorpion (TMS), Jalapeno (J), Serrano pepper (SP), Habanero Red Savina (HRS), Bhut Jolokia (BJ), and Jamaica Rosso (JR) peppers, demonstrating their inhibitory effect on the relaxation of pBR by Topo I. DNA topoisomerase II (Topo II) is proven therapeutic target of anticancer drugs. Complete inhibition of Topo II was observed for samples TMS, HR, and HMR. Extracts J and SP had the lowest capsaicin and dihydrocapsaicin content compared to other peppers. HR, HMR, TMS, J, S, HRS, BJ, JR extracts showed the anticancer effect, examined by MTS and xCell assay on the in vitro culture of human colon carcinoma cell line HCT116.

摘要

DNA 拓扑异构酶在正常细胞生长过程中调节 DNA 拓扑结构的构象变化，如复制、转录、重组和修复，并且可能成为抗癌药物的靶点。使用 DNA 拓扑结构测定法研究了来自哈瓦那红辣椒 (HR)、哈瓦那玛雅红辣椒 (HMR)、特立尼达莫鲁加蝎子辣椒 (TMS)、墨西哥胡椒 (J)、 Serrano 辣椒 (SP)、哈瓦那红 Savina 辣椒 (HRS)、印度鬼椒 (BJ) 和牙买加红辣椒 (JR) 提取物的 DNA 损伤/保护活性，证明它们对拓扑异构酶 I 松弛 pBR 的抑制作用。DNA 拓扑异构酶 II (Topo II) 是抗癌药物的既定治疗靶点。样品 TMS、HR 和 HMR 完全抑制了 Topo II。与其他辣椒相比，J 和 SP 提取物的辣椒素和二氢辣椒素含量最低。HR、HMR、TMS、J、S、HRS、BJ 和 JR 提取物在体外培养的人结肠癌细胞系 HCT116 上通过 MTS 和 xCell 测定法显示出抗癌作用。