人二噁英诱导型细胞色素P1-450：互补DNA及氨基酸序列

Human dioxin-inducible cytochrome P1-450: complementary DNA and amino acid sequence.

作者信息

Jaiswal A K, Gonzalez F J, Nebert D W

出版信息

Science. 1985 Apr 5;228(4695):80-3. doi: 10.1126/science.3838385.

Abstract

Induction of cytochrome P1-450 has been linked to susceptibility to certain chemically induced cancers in mouse and man. Treatment of the human cell line MCF-7 with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) results in high levels of aryl hydrocarbon (benzo[a]pyrene) hydroxylase (P1-450) activity. This cell line was used to isolate a human P1-450 full-length complementary DNA (cDNA) clone. The cDNA is 2566 nucleotides in length, encodes a polyadenylated messenger RNA (2.8 kilobases in length), and has a continuous reading frame producing a protein with 512 residues (molecular weight, 58,151). The human P1-450 cDNA and protein are 63 percent and 80 percent similar to mouse P1-450 cDNA and protein, respectively. Whereas the mouse TCDD-inducible P-450 gene subfamily has two members (P1-450 and P3-450), the human TCDD-inducible gene subfamily appears to have only one gene (P1-450).

摘要

细胞色素P1 - 450的诱导与小鼠和人类对某些化学诱导癌症的易感性有关。用2,3,7,8 - 四氯二苯并 - p - 二恶英（TCDD）处理人细胞系MCF - 7会导致高水平的芳烃（苯并[a]芘）羟化酶（P1 - 450）活性。该细胞系被用于分离人P1 - 450全长互补DNA（cDNA）克隆。该cDNA长度为2566个核苷酸，编码一个聚腺苷酸化的信使RNA（长度为2.8千碱基），并具有一个连续的阅读框，产生一个含有512个残基的蛋白质（分子量为58,151）。人P1 - 450 cDNA和蛋白质分别与小鼠P1 - 450 cDNA和蛋白质有63%和80%的相似性。虽然小鼠TCDD诱导型P - 450基因亚家族有两个成员（P1 - 450和P3 - 450），但人TCDD诱导型基因亚家族似乎只有一个基因（P1 - 450）。