一种用于检测马血浆中孕酮的酶免疫测定法（EIA）。

An enzyme immunoassay (EIA) for progesterone in horse plasma.

作者信息

Seeger K, Thurow H, Haede W, Knapp E

出版信息

J Immunol Methods. 1979;28(3-4):211-7. doi: 10.1016/0022-1759(79)90188-1.

Abstract

A simple enzyme immunoassay (EIA) for the measurement of progesterone is described. Antibody against 11-OH-hemisuccinate-BSA is bound to polystyrene tubes. 11-OH-hemisuccinyl-beta-D-galactosidase is used as enzyme-coupled antigen and methylumbelliferyl-beta-D-galactoside as substrate. Concentrations down to 0.156 ng/ml plasm or amounts of 93 pg/tube are detectable. Probit analysis gave a linear relationship between log concentration and percentage of binding. A comparison of EIA and radioimmunoassay gave a correlation coefficient of 0.81. The assay is sufficiently sensitive to estimate progesterone levels in plasma.

摘要

本文描述了一种用于测定孕酮的简单酶免疫测定法（EIA）。抗11-OH-半琥珀酸-BSA抗体结合到聚苯乙烯管上。11-OH-半琥珀酰-β-D-半乳糖苷酶用作酶偶联抗原，甲基伞形酮基-β-D-半乳糖苷用作底物。血浆中低至0.156 ng/ml的浓度或每管93 pg的量均可检测到。概率分析得出对数浓度与结合百分比之间呈线性关系。EIA与放射免疫测定法的比较得出相关系数为0.81。该测定法灵敏度足以估计血浆中的孕酮水平。