Parham D, Whitaker J N, Berard C W
Arch Pathol Lab Med. 1985 Mar;109(3):250-5.
Cathepsin D is an acidic endopeptidase that has been found in the cytoplasm of neurons and other cells. To test the hypothesis that the cellular content or distribution of cathepsin D may serve as a marker of neuronal differentiation in childhood neoplasia, we performed an immunocytochemical study of the cathepsin D content of tumors that occurred in 49 pediatric patients. We found diffuse, moderate to heavy staining primarily in differentiating neurogenous tumors and minimal staining in one Wilms' tumor, one Ewing's sarcoma, and one rhabdomyosarcoma. Benign reactive histiocytes stained intensely in most tumors. We concluded that although the presence of cathepsin D in malignant cells is not diagnostic of neural tumors, it may serve as a marker for cellular maturation in neuroblastomas and as a marker of macrophage infiltration in human tumors.
组织蛋白酶D是一种酸性内肽酶，已在神经元和其他细胞的细胞质中被发现。为了验证组织蛋白酶D的细胞含量或分布可能作为儿童肿瘤中神经元分化标志物的假说，我们对49例儿科患者发生的肿瘤的组织蛋白酶D含量进行了免疫细胞化学研究。我们发现主要在分化中的神经源性肿瘤中有弥漫性、中度至重度染色，而在1例肾母细胞瘤、1例尤因肉瘤和1例横纹肌肉瘤中染色极少。在大多数肿瘤中，良性反应性组织细胞染色强烈。我们得出结论，虽然恶性细胞中组织蛋白酶D的存在不能诊断神经肿瘤，但它可能作为神经母细胞瘤中细胞成熟的标志物以及人类肿瘤中巨噬细胞浸润的标志物。
