College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu Province 225009, China.
College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu Province 225009, China; Joint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu Province 225009, China.
Int J Biol Macromol. 2024 Apr;263(Pt 2):130334. doi: 10.1016/j.ijbiomac.2024.130334. Epub 2024 Feb 20.
Alfalfa polysaccharide (AP) and sulfated alfalfa polysaccharide (SAP) exhibit potential for alleviating obesity. This study aimed to analyze the mechanism of action of AP and SAP in alleviating obesity through combined microbiomics and metabolomics. The research selected validated optimal AP and SAP concentration for experiment. The results showed that AP and SAP down-regulated colonic inflammatory gene expression, regulated intestinal pH to normal, and restored intestinal growth. Microbial sequencing showed that AP and SAP altered the microbial composition ratio. AP increased the relative abundance of Muribaculaceae and Romboutsia. SAP increased the relative abundance of Dubosiella, Fecalibaculum and Desulfovibrionaceae. Metabolomic analysis showed that AP regulated steroid hormone biosynthesis, neuroactive ligand-receptor interactions and bile secretion pathways. SAP focuses more on pathways related to amino acid metabolism. Meanwhile, AP and SAP down-regulated the mRNA expression of colonic COX-2, PepT-1 and HK2 and up-regulated the mRNA expression of TPH1. Correlation analysis showed a strong correlation between metabolites and gut bacteria. Dubosiella, Faecalibaculum may be the critical marker flora for polysaccharides to alleviate obesity. This study indicates that AP and SAP alleviate obesity through different pathways and that specific polysaccharide modifications affect characteristic microbial and metabolic pathways, providing new insights into polysaccharide modifications.
苜蓿多糖（AP）和硫酸化苜蓿多糖（SAP）在缓解肥胖方面具有潜力。本研究旨在通过微生物组学和代谢组学相结合的方法分析 AP 和 SAP 缓解肥胖的作用机制。研究选择了经过验证的 AP 和 SAP 的最佳实验浓度。结果表明，AP 和 SAP 下调了结肠炎症基因的表达，调节肠道 pH 值恢复正常，并恢复了肠道生长。微生物测序表明，AP 和 SAP 改变了微生物组成比例。AP 增加了 Muribaculaceae 和 Romboutsia 的相对丰度。SAP 增加了 Dubosiella、Fecalibaculum 和 Desulfovibrionaceae 的相对丰度。代谢组学分析表明，AP 调节了甾体激素生物合成、神经活性配体-受体相互作用和胆汁分泌途径。SAP 更侧重于与氨基酸代谢相关的途径。同时，AP 和 SAP 下调了结肠 COX-2、PepT-1 和 HK2 的 mRNA 表达，上调了 TPH1 的 mRNA 表达。相关性分析表明代谢物与肠道细菌之间存在很强的相关性。Dubosiella、Faecalibaculum 可能是多糖缓解肥胖的关键标记菌群。本研究表明，AP 和 SAP 通过不同途径缓解肥胖，特定的多糖修饰影响特征性微生物和代谢途径，为多糖修饰提供了新的见解。
