Guo Changzi, Shao Liyuan, Zhao Wanqin, Han Yi, Zhang Xuli
School of Environmental Science and Engineering, Shaanxi University of Science and Technology, Xi'an, 710021, China.
School of Environmental Science and Engineering, Shaanxi University of Science and Technology, Xi'an, 710021, China.
Chemosphere. 2024 Mar;352:141518. doi: 10.1016/j.chemosphere.2024.141518. Epub 2024 Feb 21.
Under the background of carbon neutrality, resource and energy utilization technologies have become the focus of future research. The paper investigated the removal efficiencies and varying characteristics of substrates and microbial community structure in the simultaneous sulfide and nitrate biological removal (SSNBR) process. The results showed that the sulfide and nitrate removal loads reached 2.998 kg m∙d and 1.011 kg m∙d respectively when HRT was 2.4 h. The sulfide and nitrate molar ratios (S/N ratios) hardly influenced the removal efficiencies of sulfide and nitrate. However, the reaction products sulfate and nitrite concentrations in the effluent became higher as the S/N ratios decreased. Under the S/N ratio of 5:5, when the influent sulfide and nitrate concentrations were improved from 100 mg L to 600 mg L and from 87.5 mg L to 306.25 mg L, respectively, the sulfide removal efficiencies were all above 99%, but the nitrate removal efficiencies reduced from 95.53% to 55.54%. Sulfide removal effect was better than nitrate. HRT had great effect on the nitrate removal efficiencies, but hardly affected the sulfide removal. When HRT was shortened from 12 h to 2.4 h, the sulfide removal efficiencies were all above 99%, while the nitrate removal efficiencies decreased from 93.14% to 77.04%. The main functional genera included Exiguobacterium, Clostridium, Bacillus, Thiobacillus and Sphingomonas, all of which had the nitrogen and sulfur removal functions.
在碳中和背景下，资源与能源利用技术已成为未来研究的重点。本文研究了同步生物去除硫化物和硝酸盐（SSNBR）过程中底物的去除效率、变化特征以及微生物群落结构。结果表明，当水力停留时间（HRT）为2.4小时时，硫化物和硝酸盐的去除负荷分别达到2.998 kg·m⁻³·d和1.011 kg·m⁻³·d。硫化物与硝酸盐的摩尔比（S/N比）对硫化物和硝酸盐的去除效率影响不大。然而，随着S/N比降低，出水反应产物硫酸盐和亚硝酸盐的浓度升高。在S/N比为5:5的情况下，当进水硫化物和硝酸盐浓度分别从100 mg·L提高到600 mg·L以及从87.5 mg·L提高到306.25 mg·L时，硫化物去除效率均高于99%，但硝酸盐去除效率从95.53%降至55.54%。硫化物去除效果优于硝酸盐。HRT对硝酸盐去除效率影响较大，但对硫化物去除影响较小。当HRT从12小时缩短至2.4小时时，硫化物去除效率均高于99%，而硝酸盐去除效率从93.14%降至77.04%。主要功能菌属包括嗜冷栖芽孢杆菌、梭菌属、芽孢杆菌属、硫杆菌属和鞘氨醇单胞菌属，它们均具有脱氮除硫功能。
