Rabbitts T H, Sims J, Smith W, Tunnacliffe A
Ann Inst Pasteur Immunol (1985). 1985 Jan-Feb;136C(1):131-41. doi: 10.1016/s0769-2625(85)80045-3.
cDNA clones encoding the beta chain of the T-cell antigen receptor from the human leukaemic cell line JM (which extend through the constant region and into the variable region of the receptor) show hybridization only to RNA from JM cells, and not in five other T-cell leukaemias. Multiple hybridizing bands were detected in genomic DNA digests by a V beta probe, suggesting that inherited V beta gene diversity will be significant. Two beta-chain constant region genes (designated C beta 1 and C beta 2) occur in human genomic DNA, and both of these genes are capable of rearrangement in T-cell DNA. When the downstream C beta 2 gene is rearranged, deletion of the C beta 1 gene can occur. These characteristics of rearrangement and deletion of beta-chain genes show analogies to the immunoglobulin gene loci.
编码来自人白血病细胞系JM的T细胞抗原受体β链的cDNA克隆（其延伸穿过受体的恒定区并进入可变区）仅与JM细胞的RNA杂交，而在其他五种T细胞白血病中不杂交。用Vβ探针在基因组DNA消化物中检测到多个杂交带，这表明遗传的Vβ基因多样性将很显著。人基因组DNA中存在两个β链恒定区基因（命名为Cβ1和Cβ2），并且这两个基因都能够在T细胞DNA中重排。当下游的Cβ2基因重排时，Cβ1基因可能会发生缺失。β链基因的这些重排和缺失特征与免疫球蛋白基因座类似。
