Ackland N R, Tannock G A, Young I F
Appl Environ Microbiol. 1985 Apr;49(4):920-4. doi: 10.1128/aem.49.4.920-924.1985.
A device for the decontamination of large numbers of infected eggs which remain as by-products of vaccine production is described. Homogenates of infected eggs were heated to 60 degrees C and maintained at that temperature for 3 h in a specially constructed recirculation tank. The infectivity of all detectable influenza viruses and more than 99% of the bacteria associated with the homogenates was destroyed by this procedure. Eggshell material was separated from the proteins present in the homogenate at the end of the heating cycle by means of a cyclone separator. The proportions of all the amino acids, except cystine, in the proteins remaining in the suspension after the heating cycle were similar to that normally present in whole embryos.
描述了一种用于净化大量作为疫苗生产副产品留存的受感染鸡蛋的装置。将受感染鸡蛋的匀浆加热至60摄氏度,并在特制的循环罐中维持该温度3小时。通过该程序可破坏所有可检测到的流感病毒以及与匀浆相关的99%以上细菌的传染性。在加热循环结束时,借助旋风分离器将蛋壳物质与匀浆中存在的蛋白质分离。加热循环后悬浮液中剩余蛋白质中除胱氨酸外的所有氨基酸比例与全胚胎中正常存在的比例相似。