Song Dong-Heon, Yang Na-Eun, Ham Youn-Kyung, Kim Hyun-Wook
Department of Animal Science & Biotechnology, Gyeongsang National University, Jinju 52725, Republic of Korea.
Department of GreenBio Science, Gyeongsang National University, Jinju 52725, Republic of Korea.
Gels. 2024 Feb 3;10(2):124. doi: 10.3390/gels10020124.
The physicochemical properties of the mixed gelatin gels with soy and whey proteins were investigated to develop the gel base with a soft texture and abundant essential amino acids for the elderly. Gelatin-only gel (control) was prepared at 6% (/), and mixed gelatin gels were formulated by replacing gelatin with soy protein isolate and whey protein concentrate at different mixing ratios [gelatin (G):soy protein isolate (S):whey protein concentrate (W)]. Results showed that replacing gelatin with the globular proteins in gelatin gels increased the pH value and processing yield ( < 0.05). Moreover, the mixed gelatin gels, particularly the G2:S1:W3 treatment, showed significantly higher essential amino acids than the gelatin-only control. The partial replacement of gelatin with the globular proteins could decrease the hardness of gelatin gel ( < 0.05), but there was no difference in hardness between the G2:G3:W1, G2:S2:W2, and G2:S1:W3 treatments ( > 0.05). The results of protein pattern, x-ray diffraction, and microstructure had no clear evidence for specific protein-protein interaction in the mixed gelatin gels. Therefore, this study indicates that mixed gelatin gels with the globular proteins at specific mixing ratios could be a practical approach to providing a soft texture and high-level essential amino acids to the elderly.
研究了含有大豆蛋白和乳清蛋白的混合明胶凝胶的物理化学性质,旨在开发一种质地柔软且富含老年人必需氨基酸的凝胶基质。以6%(/)的浓度制备纯明胶凝胶(对照),并通过以不同混合比例[明胶(G):大豆分离蛋白(S):乳清浓缩蛋白(W)]用大豆分离蛋白和乳清浓缩蛋白替代明胶来配制混合明胶凝胶。结果表明,用明胶凝胶中的球状蛋白替代明胶会提高pH值和加工产量(<0.05)。此外,混合明胶凝胶,特别是G2:S1:W3处理组,其必需氨基酸含量显著高于纯明胶对照组。用球状蛋白部分替代明胶可降低明胶凝胶的硬度(<0.05),但G2:G3:W1、G2:S2:W2和G2:S1:W3处理组之间的硬度没有差异(>0.05)。蛋白质图谱、X射线衍射和微观结构的结果没有明确证据表明混合明胶凝胶中存在特定的蛋白质-蛋白质相互作用。因此,本研究表明,以特定混合比例含有球状蛋白的混合明胶凝胶可能是一种为老年人提供柔软质地和高水平必需氨基酸的实用方法。