Analytical/Bioanalytical Chemistry & Nanotechnology Group, Department of Chemistry, University of Patras, 26504 Patras, Greece.
Hellenic Centre for Marine Research, Institute for Marine Biological Resources, 46.7 km Athens-Sounion, Mavro Lithari, Anavyssos, 19013 Attika, Greece.
Biosensors (Basel). 2024 Feb 2;14(2):82. doi: 10.3390/bios14020082.
Tuna is an excellent food product, relatively low in calories, that is recommended for a balanced diet. The continuously increasing demand, especially for bluefin-tuna-based food preparations, and its relatively high market price make adulteration by intentionally mixing with other lower-priced tunas more prospective. The development of rapid methods to detect tuna adulteration is a great challenge in food analytical science. We have thus developed a simple, fast, and low-cost molecular rapid test for the visual detection of tuna adulteration. It is the first sensor developed for tuna authenticity testing. The three species studied were (BFT), , and . DNA was isolated from fresh and heat-treated cooked fish samples followed by PCR. The PCR products were hybridized (10 min) to specific probes and applied to the rapid sensing device. The signal was observed visually in 10-15 min using gold nanoparticle reporters. The method was evaluated employing binary mixtures of PCR products from fresh tissues and mixtures of DNA isolates from heat-treated tissues (canned products) at adulteration percentages of 1-100%. The results showed that the method was reproducible and specific for each tuna species. As low as 1% of tuna adulteration was detected with the naked eye.
金枪鱼是一种卡路里含量相对较低的优质食品，推荐用于均衡饮食。不断增长的需求，特别是对基于蓝鳍金枪鱼的食品制备的需求，以及其相对较高的市场价格，使得故意与其他价格较低的金枪鱼混合掺假更具前景。开发快速方法来检测金枪鱼掺假是食品分析科学面临的巨大挑战。因此，我们开发了一种简单、快速且低成本的分子快速测试方法，用于可视化检测金枪鱼掺假。这是第一个用于金枪鱼真实性测试的传感器。研究的三个物种是（BFT）、 和 。从新鲜和热处理过的熟鱼样本中分离出 DNA，然后进行 PCR。PCR 产物与特定探针杂交（10 分钟），并应用于快速感应装置。使用金纳米粒子报告器在 10-15 分钟内观察到信号。该方法采用新鲜组织的 PCR 产物的二进制混合物和热处理组织（罐头产品）的 DNA 分离物的混合物（掺假百分比为 1-100%）进行评估。结果表明，该方法对于每种金枪鱼物种都是可重复且具有特异性的。即使金枪鱼掺假率低至 1%，也可以用肉眼检测到。
