粗糙表面增强的纳米酶活性用于 HO 和四环素检测。

Enhanced Nanozymatic Activity on Rough Surfaces for HO and Tetracycline Detection.

机构信息

Department of Food Science & Nutrition, College of Food and Agricultural Sciences, King Saud University, Riyadh 11451, Saudi Arabia.

出版信息

Biosensors (Basel). 2024 Feb 17;14(2):106. doi: 10.3390/bios14020106.

DOI:10.3390/bios14020106

PMID:38392024

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10886513/

Abstract

The needless use of tetracyclines (TCs) in foodstuffs is a huge health concern in low- and middle-income and Arab countries. Herein, a sensitive and faster monitoring system for HO and TCs is proposed, utilizing the large surface-to-volume ratio of a non-spherical gold nanoparticle/black phosphorus nanocomposite (BP-nsAu NPs) for the first time. BP-nsAu NPs were synthesized through a single-step method that presented nanozymatic activity through 3,3',5,5'-Tetramethylbenzidine (TMB) oxidation while HO was present and obeyed the Michaelis-Menten equation. The nanozymatic activity of the BP-nsAu NPs was enhanced 12-fold and their detection time was decreased 83-fold compared to conventional nanozymatic reactions. The proposed method enabled us to quantify HO with a limit of detection (LOD) value of 60 nM. Moreover, target-specific aptamer-conjugated BP-nsAu NPs helped us detect TCs with an LOD value of 90 nM. The present strategy provides a proficient route for low-level TC monitoring in real samples.

摘要

在中低收入国家和阿拉伯国家，食物中不必要地使用四环素（TCs）是一个巨大的健康隐患。在此，首次提出了一种利用非球形金纳米粒子/黑磷纳米复合材料（BP-nsAu NPs）的大表面积与体积比，用于 HO 和 TCs 的更敏感和更快的监测系统。BP-nsAu NPs 通过一步法合成，当存在 HO 时，通过 3,3',5,5'-四甲基联苯胺（TMB）氧化表现出纳米酶活性，并遵循米氏方程。与传统的纳米酶反应相比，BP-nsAu NPs 的纳米酶活性提高了 12 倍，检测时间缩短了 83 倍。该方法使我们能够以 60 nM 的检测限（LOD）值定量检测 HO。此外，目标特异性适体偶联的 BP-nsAu NPs 有助于我们以 90 nM 的 LOD 值检测 TCs。本研究策略为实际样品中低水平 TC 监测提供了一种有效的途径。