College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan 430070, China.
Biosensors (Basel). 2024 Mar 31;14(4):169. doi: 10.3390/bios14040169.
The peroxidase-like behaviors of gold nanoparticles (AuNPs) have the potential to the development of rapid and sensitive colorimetric assays for specific food ingredients and contaminants. Here, using NaBH as a reducing agent, AuNPs with a supramolecular macrocyclic compound β-cyclodextrin (β-CD) capped were synthesized under alkaline conditions. Monodispersal of β-CD@AuNPs possessed a reduction in diameter size and performed great peroxidase-like activities toward both substrates, HO and TMB. In the presence of HO, the color change of TMB oxidization to oxTMB was well-achieved using β-CD@AuNPs as the catalyst, which was further employed to develop colorimetric assays for ascorbic acid, with a limit of detection as low as 0.2 μM in ddHO. With the help of the host-guest interaction between β-CD and adamantane, AuNPs conjugated with nanobodies to exhibit peroxidase-like activities and specific recognition against Typhimurium simultaneously. Based on this bifunctional bioprobe, a selective and sensitive one-step colorimetric assay for . Typhimurium was developed with a linear detection from 8.3 × 10 to 2.6 × 10 CFU/mL and can be provided to spiked lettuce with acceptable recoveries of 97.31% to 103.29%. The results demonstrated that the excellent peroxidase-like behaviors of β-CD@AuNPs can be applied to develop a colorimetric sensing platform in the food industry.
金纳米粒子（AuNPs）的过氧化物酶样行为有可能开发出用于特定食品成分和污染物的快速灵敏比色分析方法。在这里，我们使用 NaBH4 作为还原剂，在碱性条件下合成了具有超分子大环化合物β-环糊精（β-CD）封端的 AuNPs。β-CD@AuNPs 的单分散性具有直径尺寸减小的特点，并对两种底物 HO 和 TMB 表现出优异的过氧化物酶样活性。在 HO 的存在下，使用β-CD@AuNPs 作为催化剂可以很好地实现 TMB 氧化为 oxTMB 的颜色变化，进一步用于开发比色法测定抗坏血酸，在 ddHO 中的检测限低至 0.2 μM。借助β-CD 和金刚烷之间的主客体相互作用，AuNPs 与纳米抗体结合，同时表现出过氧化物酶样活性和对鼠伤寒沙门氏菌的特异性识别。基于这种双功能生物探针，开发了一种用于鼠伤寒沙门氏菌的选择性和灵敏的一步比色分析方法，线性检测范围为 8.3×10 到 2.6×10 CFU/mL，可以对添加的生菜进行检测，回收率为 97.31%到 103.29%。结果表明，β-CD@AuNPs 的优异过氧化物酶样行为可应用于食品工业中开发比色传感平台。
