The Role of Rv1476 in Regulating Stress Response and Intracellular Survival of .

The virulence of () is related to many factors, including intracellular survival, cell wall permeability, and cell envelope proteins. However, the biological function of the membrane protein remains unclear. To investigate the potential role played by , we constructed an overexpression strain and found that overexpression of enhanced the intracellular survival of , while having no impact on the growth rate in vitro. Stress experiments demonstrated that the overexpression strain displayed increased susceptibility to different stresses compared to the wild-type strain. Transcriptome analysis showed that overexpression causes changes in the transcriptome of THP-1 cells, and differential genes are mainly enriched in cell proliferation, fatty acid degradation, cytokine-cytokine receptor interaction, and immune response pathways. overexpression inhibited the expression of some anti-tuberculosis-related genes, such as , , , , , , , , and . Altogether, we conclude that may play a critical role for in macrophage survival.