Gasson J C, Golde D W, Kaufman S E, Westbrook C A, Hewick R M, Kaufman R J, Wong G G, Temple P A, Leary A C, Brown E L
Nature. 1985;315(6022):768-71. doi: 10.1038/315768a0.
Erythropoietin is the primary physiological regulator of erythropoiesis; however, in vitro studies have identified another class of mediators which appear to be important in stimulating erythroid progenitors. These factors have generally been referred to as burst-promoting activities (BPA), because they stimulate the growth of early erythroid progenitors referred to as burst-forming units-erythroid (BFU-E) which give rise to colonies of up to thousands of haemoglobinized cells. We recently reported purification of a burst-promoting activity from medium conditioned by the Mo T-lymphoblast cell line infected with human T-cell lymphotropic virus type II (HTLV-II). This purified glycoprotein of relative molecular mass (Mr) 28,000 also stimulates colony formation by more mature erythroid precursors (CFU-E) and is therefore referred to as erythroid-potentiating activity (EPA). Purified EPA specifically stimulates human and murine cells of the erythroid lineage, unlike murine interleukin-3 (IL-3) which stimulates precursor cells from all haematopoietic lineages. We report here the isolation of a complementary DNA molecular clone encoding EPA and its use in producing EPA in COS (monkey) cells and CHO (Chinese hamster ovary) cells. We also define the organization of the EPA gene in human DNA.
促红细胞生成素是红细胞生成的主要生理调节因子；然而，体外研究已鉴定出另一类介质，它们似乎在刺激红系祖细胞方面很重要。这些因子通常被称为爆式促进活性（BPA），因为它们刺激早期红系祖细胞（称为爆式红系集落形成单位，BFU-E）的生长，这些祖细胞可形成多达数千个血红蛋白化细胞的集落。我们最近报道了从感染人II型嗜T淋巴细胞病毒（HTLV-II）的Mo T淋巴细胞系条件培养基中纯化出一种爆式促进活性物质。这种相对分子质量（Mr）为28,000的纯化糖蛋白还能刺激更成熟的红系前体细胞（CFU-E）形成集落，因此被称为红系增强活性（EPA）。纯化的EPA特异性刺激红系谱系的人和小鼠细胞，这与刺激所有造血谱系前体细胞的小鼠白细胞介素-3（IL-3）不同。我们在此报告编码EPA的互补DNA分子克隆的分离及其在COS（猴）细胞和CHO（中国仓鼠卵巢）细胞中产生EPA的用途。我们还确定了人DNA中EPA基因的结构。
