Department of Entomology, Michigan State University, East Lansing, Michigan, United States of America.
Department of Entomology, Purdue University, West Lafayette, Indiana, United States of America.
PLoS One. 2024 Feb 23;19(2):e0295928. doi: 10.1371/journal.pone.0295928. eCollection 2024.
The fall armyworm (Spodoptera frugiperda) is one of the most destructive pests of corn. New infestations have been reported in the East Hemisphere, reaching India, China, Malaysia, and Australia, causing severe destruction to corn and other crops. In Puerto Rico, practical resistance to different mode of action compounds has been reported in cornfields. In this study, we characterized the inheritance of resistance to chlorantraniliprole and flubendiamide and identified the possible cross-resistance to cyantraniliprole and cyclaniliprole. The Puerto Rican (PR) strain showed high levels of resistance to flubendiamide (RR50 = 2,762-fold) and chlorantraniliprole (RR50 = 96-fold). The inheritance of resistance showed an autosomal inheritance for chlorantraniliprole and an X-linked inheritance for flubendiamide. The trend of the dominance of resistance demonstrated an incompletely recessive trait for H1 (♂ SUS × ♀ PR) × and an incompletely dominant trait for H2 (♀ SUS × ♂ PR) × for flubendiamide and chlorantraniliprole. The PR strain showed no significant presence of detoxification enzymes (using synergists: PBO, DEF, DEM, and VER) to chlorantraniliprole; however, for flubendiamide the SR = 2.7 (DEM), SR = 3.2 (DEF) and SR = 7.6 (VER) indicated the role of esterases, glutathione S- transferases and ABC transporters in the metabolism of flubendiamide. The PR strain showed high and low cross-resistance to cyantraniliprole (74-fold) and cyclaniliprole (11-fold), respectively. Incomplete recessiveness might lead to the survival of heterozygous individuals when the decay of diamide residue occurs in plant tissues. These results highlight the importance of adopting diverse pest management strategies, including insecticide rotating to manage FAW populations in Puerto Rico and other continents.
秋粘虫（Spodoptera frugiperda）是玉米的最具破坏性害虫之一。新的疫情已在东半球报告，涉及印度、中国、马来西亚和澳大利亚，对玉米和其他作物造成严重破坏。在波多黎各，据报道玉米田对不同作用模式化合物具有实际抗性。在这项研究中，我们对氯虫苯甲酰胺和氟苯虫酰胺的抗性遗传进行了特征描述，并确定了对氰虫酰胺和环丙虫酰胺可能的交叉抗性。波多黎各（PR）品系对氟苯虫酰胺（RR50 = 2762 倍）和氯虫苯甲酰胺（RR50 = 96 倍）表现出高水平的抗性。抗性遗传表现为氯虫苯甲酰胺的常染色体遗传和氟苯虫酰胺的 X 连锁遗传。抗性的显性趋势表明，氟苯虫酰胺对 H1（♂SUS×♀PR）×表现为不完全隐性性状，对 H2（♀SUS×♂PR）×表现为不完全显性性状；而对氯虫苯甲酰胺则无显著的解毒酶（用增效剂：PBO、DEF、DEM 和 VER）存在；然而，对于氟苯虫酰胺，SR = 2.7（DEM）、SR = 3.2（DEF）和 SR = 7.6（VER）表明酯酶、谷胱甘肽 S-转移酶和 ABC 转运体在氟苯虫酰胺的代谢中起作用。PR 品系对氰虫酰胺（74 倍）和环丙虫酰胺（11 倍）表现出高和低的交叉抗性。不完全隐性可能导致杂合个体在植物组织中双酰胺残留衰减时存活。这些结果强调了采用多样化的害虫管理策略的重要性，包括杀虫剂轮换，以管理波多黎各和其他大陆的秋粘虫种群。
