Stelzer K J, Gordon M A
Chem Biol Interact. 1985 Jun;54(1):105-16. doi: 10.1016/s0009-2797(85)80156-3.
Pyrethroid interactions with dipalmitoyl phosphatidylcholine (DPPC) vesicles have been characterized in bilayers having large and small radii of curvature. The abilities of pyrethroids to alter the gel-fluid phase transition profiles were determined by steady state fluorescence anisotropy and phase-modulation lifetime techniques using the fluorescent probes cis- and trans-parinaric acid. Using the geometric isomers of parinaric acid as membrane probes, pyrethroids were found to lower the phase transition temperature (Tc) of DPPC large multilamellar vesicles with the same order of comparative effectiveness as previously reported using the fluorescent probe 1,6-diphenyl-1,3,5-hexatriene (DPH). Permethrin had a greater depressive effect upon the Tc of DPPC in the small unilamellar vesicle (SUV) system than in the large multilamellar system. Conversely, allethrin was less effective in reducing the Tc of DPPC SUVs. The enhanced effect of permethrin in decreasing the Tc of DPPC SUVs was greatest in regions of more rigid lipid packing, as determined by trans-parinaric acid fluorescence parameters. The results indicate that changes in lipid packing configuration caused by differing bilayer radii of curvature may alter the interactive characteristics of pyrethroids with lipid membranes.
拟除虫菊酯与二棕榈酰磷脂酰胆碱（DPPC）囊泡的相互作用已在具有大曲率半径和小曲率半径的双层膜中得到表征。使用顺式和反式十八碳四烯酸荧光探针，通过稳态荧光各向异性和相位调制寿命技术测定了拟除虫菊酯改变凝胶-流体相转变曲线的能力。以十八碳四烯酸的几何异构体作为膜探针，发现拟除虫菊酯降低了DPPC大单层囊泡的相转变温度（Tc），其相对有效性顺序与先前使用荧光探针1,6-二苯基-1,3,5-己三烯（DPH）报道的相同。在小单层囊泡（SUV）系统中，氯菊酯对DPPC的Tc的抑制作用比对大单层囊泡系统中的更强。相反，丙烯菊酯在降低DPPC SUVs的Tc方面效果较差。根据反式十八碳四烯酸荧光参数确定，在脂质堆积更紧密的区域，氯菊酯降低DPPC SUVs的Tc的增强作用最为显著。结果表明，由不同双层曲率半径引起的脂质堆积构型变化可能会改变拟除虫菊酯与脂质膜的相互作用特性。
