Milojević Lazar, Velebit Branko, Janković Vesna, Mitrović Radmila, Betić Nikola, Simunović Sara, Dimitrijević Mirjana
Institute of Meat Hygiene and Technology, Kaćanskog 13, 11040 Belgrade, Serbia.
Faculty of Veterinary Medicine, University of Belgrade, Bulevar Oslobođenja 18, 11000 Belgrade, Serbia.
Animals (Basel). 2024 Feb 10;14(4):586. doi: 10.3390/ani14040586.
The goal of this study conducted in Serbia was to detect HEV in pig liver samples from slaughterhouses, retail outlets, and environmental swabs over the course of a year. All positive HEV samples were measured and expressed as HEV gene copy numbers per gram of sample, and a representative number of samples were sequenced using the Sanger approach. A total of 45 HEV-positive samples were re-amplified using nested RT-PCR employing CODEHOP primers targeting ORF2 (493 nucleotides). The average prevalence of the HEV genotype 3 in all pig liver samples from the slaughterhouses was 29%, while HEV prevalence was 44% in liver samples from animals younger than 3 months. HEV RNA was found in thirteen out of sixty (22%) environmental swab samples that were taken from different surfaces along the slaughter line. Our findings confirmed seasonal patterns in HEV prevalence, with two picks (summer and winter periods) during the one-year examination. Among HEV-positive samples, the average viral particles for all positive liver samples was 4.41 ± 1.69 log genome copies per gram. Phylogenetic analysis revealed the majority of HEV strains (43/45) from Serbia were grouped in the HEV-3a subtype, while two strains were classified into the HEV-3c subtype, and one strain could not be classified into any of the HEV-3 subtypes.
在塞尔维亚开展的这项研究的目的是，在一年的时间里检测来自屠宰场、零售点的猪肝样本以及环境拭子中的戊型肝炎病毒（HEV）。对所有HEV阳性样本进行测量，并以每克样本中的HEV基因拷贝数表示，使用桑格测序法对代表性数量的样本进行测序。使用靶向ORF2（493个核苷酸）的CODEHOP引物，通过巢式逆转录聚合酶链反应（RT-PCR）对总共45个HEV阳性样本进行再次扩增。屠宰场所有猪肝样本中戊型肝炎病毒3型的平均流行率为29%，而3个月龄以下动物的肝脏样本中HEV流行率为44%。在从屠宰线不同表面采集的60个环境拭子样本中，有13个（22%）检测到HEV RNA。我们的研究结果证实了HEV流行的季节性模式，在为期一年的检测中有两个高峰（夏季和冬季）。在HEV阳性样本中，所有阳性肝脏样本的平均病毒颗粒数为每克4.41±1.69 log基因组拷贝。系统发育分析显示，来自塞尔维亚的大多数HEV毒株（43/45）归为HEV-3a亚型，有两个毒株归为HEV-3c亚型，还有一个毒株无法归入任何HEV-3亚型。
