Lupulović Diana, Gnjatović Marija, Prodanov-Radulović Jasna, Ćujić Danica, Gajdov Vladimir, Samojlović Milena, Petrović Tamaš
Institute for the Application of Nuclear Energy INEP, Banatska 31b, 11080 Zemun, Serbia.
Scientific Veterinary Institute "Novi Sad", Rumenački put 20, 21000 Novi Sad, Serbia.
Animals (Basel). 2025 Jan 9;15(2):151. doi: 10.3390/ani15020151.
Hepatitis E virus (HEV) is the causative agent of acute hepatitis E infection in humans. Two epidemiological patterns of the disease exist-endemic and sporadic. Genotypes 1 (HEV-1) and 2 (HEV-2) are transmitted through contaminated water and are responsible for the outbreaks of many large-scale epidemics in developing countries of Asia and Africa. Genotypes 3 (HEV-3) and 4 (HEV-4) have zoonotic potential and cause sporadic cases and small outbreaks in high-income countries. The first case of HEV infection in swine was confirmed in 1997 and later detected in other animal species. The aim of this study was to investigate the seroprevalence of HEV infection in pig farms in Vojvodina province, Serbia. Three hundred blood samples were collected from five different categories of pigs from 3 different farms on the territory of the South Bačka district in Vojvodina (Serbia). The analyses were conducted by in-house ELISA, while the western blot method was used as a confirmatory test for doubtful results. The presence of HEV IgG was detected on all three examined farms. The established seroprevalence in Farm A was 37%, 31% in Farm B, and 54% in Farm C. The mean seroprevalence for all farms was 40.66%. A higher seroprevalence was found in fatteners compared to younger categories of pigs. We concluded that HEV is widespread on pig farms with intensive management. Further analyses should be conducted with the aim of implementing a surveillance program to prevent possible human infection.
戊型肝炎病毒（HEV）是人类急性戊型肝炎感染的病原体。该疾病存在两种流行模式——地方性流行和散发性。1型（HEV-1）和2型（HEV-2）通过受污染的水传播，是亚洲和非洲发展中国家许多大规模疫情爆发的原因。3型（HEV-3）和4型（HEV-4）具有人畜共患病潜力，在高收入国家导致散发病例和小规模疫情。1997年首次确诊猪感染HEV，后来在其他动物物种中也检测到。本研究的目的是调查塞尔维亚伏伊伏丁那省养猪场中HEV感染的血清流行率。从伏伊伏丁那（塞尔维亚）南巴奇卡地区3个不同农场的5种不同类别的猪中采集了300份血样。分析采用内部酶联免疫吸附测定（ELISA）进行，而蛋白质印迹法用作可疑结果的确证试验。在所有3个受检农场均检测到HEV IgG的存在。A农场确定的血清流行率为37%，B农场为31%，C农场为54%。所有农场的平均血清流行率为40.66%。与较年轻类别的猪相比，育肥猪的血清流行率更高。我们得出结论，在集约化管理的养猪场中HEV广泛存在。应进一步开展分析，以实施监测计划，预防可能的人类感染。
