Fernie J M, McLean A, Lamb D
Arch Pathol Lab Med. 1985 Sep;109(9):843-8.
The study objectives were to determine the effect of several factors related to tissue preparation on the relationship between medial area and size of the muscular pulmonary artery (defined in terms of total length of internal elastic lamina). This relationship took the form y = Axb that was linearized by plotting the square root of medial area against artery size. Complete distention of pulmonary arteries by an injection medium caused the internal elastic lamina to stretch by a factor of approximately 1.5. The relationship between medial area and artery size was unaffected by either the pressure method used for lung inflation/fixation or the tissue-embedding medium. Tissue shrinkage was considerable with paraffin embedding and negligible with glycol methacrylate. More arteries were considered measurable in glycol methacrylate-embedded tissue.
本研究的目的是确定与组织制备相关的几个因素对肺肌性动脉内侧面积与大小（以内弹性膜总长度定义）之间关系的影响。这种关系呈y = Axb的形式，通过绘制内侧面积的平方根与动脉大小的关系图将其线性化。注射介质使肺动脉完全扩张，导致内弹性膜伸展约1.5倍。内侧面积与动脉大小之间的关系不受用于肺充气/固定的压力方法或组织包埋介质的影响。石蜡包埋时组织收缩相当明显，而甲基丙烯酸乙二醇酯包埋时收缩可忽略不计。在甲基丙烯酸乙二醇酯包埋的组织中，可测量的动脉更多。
