Posgrado en Ciencias Genómicas, Universidad Autónoma de la Ciudad de México, Mexico City C.P. 03100, Mexico.
Licenciatura Ciencias Genómicas, Universidad Autónoma de la Ciudad de México, Mexico City C.P. 03100, Mexico.
Genes (Basel). 2024 Feb 2;15(2):201. doi: 10.3390/genes15020201.
, the causative agent of amebiasis, is the third leading cause of death among parasitic diseases globally. Its life cycle includes encystation, which has been mostly studied in , responsible for reptilian amebiasis. However, the molecular mechanisms underlying this process are not fully understood. Therefore, we focused on the identification and characterization of Myb proteins, which regulate the expression of encystation-related genes in various protozoan parasites. Through bioinformatic analysis, we identified 48 genes in encoding MYB-domain-containing proteins. These were classified into single-repeat 1R (20), 2R-MYB proteins (27), and one 4R-MYB protein. The in-silico analysis suggests that these proteins are multifunctional, participating in transcriptional regulation, chromatin remodeling, telomere maintenance, and . Transcriptomic data analysis revealed expression signatures of genes, suggesting a potential orchestration in the regulation of early and late encystation-excystation genes. Furthermore, we identified probable target genes associated with reproduction, the meiotic cell cycle, ubiquitin-dependent protein catabolism, and endosomal transport. In conclusion, our findings suggest that Myb proteins regulate stage-specific proteins and a wide array of cellular processes. This study provides a foundation for further exploration of the molecular mechanisms governing encystation and unveils potential targets for therapeutic intervention in amebiasis.
溶组织内阿米巴（Entamoeba histolytica）是引起阿米巴病的病原体，是全球寄生虫病死亡的第三大原因。其生命周期包括囊化，这在引起爬行动物阿米巴病的 中得到了广泛研究。然而，这一过程的分子机制尚不完全清楚。因此，我们专注于鉴定和表征调节各种原生动物寄生虫囊化相关基因表达的 Myb 蛋白。通过生物信息学分析，我们在 中鉴定出 48 个编码 MYB 结构域蛋白的基因。这些基因分为单重复 1R（20 个）、2R-MYB 蛋白（27 个）和一个 4R-MYB 蛋白。计算机分析表明这些蛋白具有多功能性，参与转录调控、染色质重塑、端粒维持和细胞凋亡。转录组数据分析揭示了 个基因的表达特征，表明在早期和晚期囊化-囊泡形成基因的调控中存在潜在的协调作用。此外，我们还鉴定出可能与生殖、减数细胞周期、泛素依赖性蛋白降解和内体运输相关的靶基因。总之，我们的研究结果表明 Myb 蛋白调节特定阶段的蛋白和广泛的细胞过程。这项研究为进一步探索囊化的分子机制提供了基础，并揭示了阿米巴病治疗干预的潜在靶点。
