Catanzaro D F, O'Connell A M, Morris B J
Clin Exp Pharmacol Physiol. 1985 May-Jun;12(3):295-7. doi: 10.1111/j.1440-1681.1985.tb02648.x.
A 14 kilo-base pair DNA clone (lambda HCMHC8) was isolated from a human genomic library by hybridization with a complementary DNA coding for a rabbit cardiac myosin heavy chain. lambda HCMHC8 hybridized to RNA isolated from cardiac but not skeletal muscle and formed heteroduplexes with a genomic clone coding for the fast type of rabbit cardiac myosin heavy chain. lambda HCMHC8 represented at least the 3' half of the gene and contained over 11 exons which together spanned 4 kb of the coding region estimated to be 6 kb. Probes made from lambda HCMHC8 were used to rescreen the library in order to isolate overlapping clones and so extend the sequence (estimated to be approximately 25 kb for the whole gene, including introns). In addition, a clone having a different restriction map was isolated suggesting that man, like rat and rabbit, has two cardiac myosin heavy chain genes. These may code for proteins having different ATPase activity and be expressed in different proportions in different cardiac states, including hypertension.
通过与编码兔心肌肌球蛋白重链的互补DNA杂交，从人基因组文库中分离出一个14千碱基对的DNA克隆（λHCMHC8）。λHCMHC8与从心脏而非骨骼肌中分离的RNA杂交，并与编码兔快型心肌肌球蛋白重链的基因组克隆形成异源双链体。λHCMHC8至少代表该基因的3'端一半，包含超过11个外显子，它们共同跨越了估计为6 kb的编码区域中的4 kb。用λHCMHC8制备的探针重新筛选文库，以分离重叠克隆，从而扩展序列（整个基因估计约为25 kb，包括内含子）。此外，还分离出一个具有不同限制性图谱的克隆，这表明人类与大鼠和兔子一样，有两个心肌肌球蛋白重链基因。这些基因可能编码具有不同ATP酶活性的蛋白质，并在包括高血压在内的不同心脏状态下以不同比例表达。
