Markantes Georgios K, Panagodimou Evangelia, Koika Vasiliki, Mamali Irene, Kaponis Apostolos, Adonakis George, Georgopoulos Neoklis A
Division of Endocrinology, Department of Internal Medicine, School of Health Sciences, University of Patras, 26504 Patras, Greece.
Department of Obstetrics and Gynecology, School of Health Sciences, University of Patras, 26504 Patras, Greece.
Biomedicines. 2024 Feb 1;12(2):334. doi: 10.3390/biomedicines12020334.
Current research suggests that polycystic ovary syndrome (PCOS) might originate in utero and implicates the placenta in its pathogenesis. Kisspeptin (KISS1) and neurokinin B (NKB) are produced by the placenta in high amounts, and they have been implicated in several pregnancy complications associated with placental dysfunction. However, their placental expression has not been studied in PCOS. We isolated mRNA after delivery from the placentae of 31 PCOS and 37 control women with term, uncomplicated, singleton pregnancies. The expression of KISS1, NKB, and neurokinin receptors 1, 2, and 3 was analyzed with real-time polymerase chain reaction, using β-actin as the reference gene. Maternal serum and umbilical cord levels of total testosterone, sex hormone-binding globulin (SHBG), free androgen index (FAI), androstenedione, dehydroepiandrosterone sulfate (DHEAS), Anti-Mullerian hormone (AMH), and estradiol were also assessed. placental mRNA expression was higher in PCOS women versus controls in pregnancies with female offspring. expression depended on fetal gender, being higher in pregnancies with male fetuses, regardless of PCOS. was positively correlated with umbilical cord FAI and AMH, and was positively correlated with cord testosterone and FAI; there was also a strong positive correlation between and expression. Women with PCOS had higher serum AMH and FAI and lower SHBG than controls. Our findings indicate that NKB might be involved in the PCOS-related placental dysfunction and warrant further investigation. Studies assessing the placental expression of should take fetal gender into consideration.
目前的研究表明，多囊卵巢综合征（PCOS）可能起源于子宫内，并且其发病机制与胎盘有关。 Kisspeptin（KISS1）和神经激肽B（NKB）由胎盘大量产生，它们与几种与胎盘功能障碍相关的妊娠并发症有关。然而，它们在PCOS患者胎盘中的表达尚未得到研究。我们从31例患有PCOS的孕妇和37例足月、无并发症、单胎妊娠的对照孕妇的胎盘中分离出产后mRNA。以β-肌动蛋白为参照基因，采用实时聚合酶链反应分析KISS1、NKB以及神经激肽受体1、2和3的表达。同时还评估了孕妇血清和脐带血中总睾酮、性激素结合球蛋白（SHBG）、游离雄激素指数（FAI）、雄烯二酮、硫酸脱氢表雄酮（DHEAS）、抗苗勒管激素（AMH）和雌二醇的水平。在怀有女性后代的妊娠中，PCOS患者胎盘mRNA表达高于对照组。表达取决于胎儿性别，无论是否患有PCOS，怀有男性胎儿的妊娠中表达更高。与脐带血FAI和AMH呈正相关，与脐带血睾酮和FAI呈正相关；与的表达之间也存在很强的正相关。患有PCOS的女性血清AMH和FAI高于对照组，而SHBG低于对照组。我们的研究结果表明，NKB可能参与了PCOS相关的胎盘功能障碍，值得进一步研究。评估表达的研究应考虑胎儿性别。
