Markopoulos Georgios, Lampri Evangeli, Tragani Ioulia, Kourkoumelis Nikolaos, Vartholomatos Georgios, Seretis Konstantinos
Haematology Laboratory-Unit of Molecular Biology and Translational Flow Cytometry, University Hospital of Ioannina, 45110 Ioannina, Greece.
Department of Pathology, Medical School, University of Ioannina, 45110 Ioannina, Greece.
Cancers (Basel). 2024 Feb 6;16(4):682. doi: 10.3390/cancers16040682.
Non-melanoma skin cancer (NMSC) is the most prevalent cancer in humans, with a high global incidence. We present a prospective clinical feasibility study on the use of intraoperative flow cytometry (iFC) for the instant diagnosis of NMSC and its complete surgical clearance. Flow cytometry, a laser-based technique, quantifies cell features, which has applications in cancer research. This study aim is to explore the potential applicability of iFC in detecting and characterizing NMSC and its surgical margins. In total, 30 patients who underwent diagnosis for NMSC were recruited. The method demonstrated high sensitivity (95.2%) and specificity (87.1%), with an accuracy of 91.1%, as confirmed with a receiver operating characteristic curve analysis. The results also indicated that most tumors were diploid, with two cases being hypoploid. The average G0/G1 fractions for normal and tumor tissue samples were 96.03 ± 0.30% and 88.03 ± 1.29%, respectively, with the tumor index escalating from 3.89 ± 0.30% to 11.95 ± 1.29% in cancerous cells. These findings underscore iFC's capability for precise intraoperative NMSC characterization and margin evaluation, promising enhanced complete tumor excision rates. Given the technique's successful application in various other malignancies, its implementation in NMSC diagnosis and treatment holds significant promise and warrants further research in clinical trials.
非黑色素瘤皮肤癌（NMSC）是人类中最常见的癌症，全球发病率很高。我们开展了一项前瞻性临床可行性研究，探讨术中流式细胞术（iFC）用于NMSC即时诊断及其手术彻底切除的情况。流式细胞术是一种基于激光的技术，可对细胞特征进行量化，在癌症研究中有应用。本研究旨在探索iFC在检测和表征NMSC及其手术切缘方面的潜在适用性。总共招募了30例接受NMSC诊断的患者。经受试者工作特征曲线分析证实，该方法显示出高灵敏度（95.2%）和特异性（87.1%），准确率为91.1%。结果还表明，大多数肿瘤为二倍体，有两例为亚二倍体。正常组织和肿瘤组织样本的平均G0/G1期比例分别为96.03±0.30%和88.03±1.29%，癌细胞中的肿瘤指数从3.89±0.30%升至11.95±1.29%。这些发现强调了iFC在术中精确表征NMSC和评估切缘的能力，有望提高肿瘤完全切除率。鉴于该技术在其他多种恶性肿瘤中的成功应用，其在NMSC诊断和治疗中的应用具有重大前景，值得在临床试验中进一步研究。
