Anderson F H, Patterson C, Burton A F
JPEN J Parenter Enteral Nutr. 1985 Jul-Aug;9(4):477-9. doi: 10.1177/0148607185009004477.
Rats were fed liquid diets for 7 days containing either triolein or Liposyn, which is rich in linoleic acid, as fat sources, and liver cell suspensions were prepared following collagenase perfusion. The release from isolated cells of alkaline phosphatase and aspartate transaminase during a 3-hr incubation did not differ. The uptake and release of 14C-taurocholate during a brief incubation was lower but not significantly in Liposyn-fed rats (0.1 greater than p greater than 0.05): the uptake was 9.74 +/- 1.58 vs 16.7 +/- 3.3 nmol/mg protein in triolein-fed rats; the release was 3.17 +/- 0.65 vs 5.35 +/- 1.01 nmol/mg protein in triolein-fed rats. The uptake of 14C-aminolevulinic acid was similar in both groups, but release of 14C-bilirubin during a 30-min incubation was 5,420 +/- 1010 in the Liposyn group vs 12,030 +/- 2,200 dpm/mg protein in the triolein group (p = 0.02). It is concluded that a diet high in linoleic acid decreases bilirubin release in isolated liver cells consistent with the ability of this diet to cause cholestasis in vivo.
给大鼠喂食含三油酸甘油酯或富含亚油酸的脂质乳剂（Liposyn）作为脂肪源的液体饮食7天，然后在胶原酶灌注后制备肝细胞悬液。在3小时孵育期间，碱性磷酸酶和天冬氨酸转氨酶从分离细胞中的释放没有差异。在短暂孵育期间，脂质乳剂喂养的大鼠中14C-牛磺胆酸盐的摄取和释放较低，但差异不显著（0.1＞p＞0.05）：摄取量在三油酸甘油酯喂养的大鼠中为16.7±3.3 nmol/mg蛋白质，而在脂质乳剂喂养的大鼠中为9.74±1.58 nmol/mg蛋白质；释放量在三油酸甘油酯喂养的大鼠中为5.35±1.01 nmol/mg蛋白质，而在脂质乳剂喂养的大鼠中为3.17±0.65 nmol/mg蛋白质。两组中14C-氨基乙酰丙酸的摄取相似，但在30分钟孵育期间，脂质乳剂组中14C-胆红素的释放为5420±1010 dpm/mg蛋白质，而三油酸甘油酯组中为12030±2200 dpm/mg蛋白质（p = 0.02）。结论是，富含亚油酸的饮食可降低分离肝细胞中胆红素的释放，这与该饮食在体内引起胆汁淤积的能力一致。
