Institute of Plant Virology, College of Plant Protection, Fujian Agriculture and Forestry University, Fujian 350002, China.
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Plant Virology, Ningbo University, Ningbo 315211, China.
Viruses. 2024 Feb 12;16(2):283. doi: 10.3390/v16020283.
Chinese bayberry () is an economically significant fruit tree native to eastern Asia and widely planted in south-central China. However, studies about the viruses infecting remain largely lacking. In the present study, we employed the metatranscriptomic method to identify viruses in leaves exhibiting yellowing and irregular margin symptoms collected in Fuzhou, a city located in China's Fujian province in the year 2022. As a consequence, a novel member of the genus was identified and tentatively named "Myrica rubra associated totivirus 1" (MRaTV1). The genome sequencing of MRaTV1 was determined by overlapping reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). The two deduced proteins encoded by MRaTV1 have the highest amino acid (aa) sequence identity to the coat protein (CP) and RNA-dependent RNA polymerase (RdRP) of Panax notoginseng virus A (PNVA), a member of the genus within the family , at 49.7% and 61.7%, respectively. According to the results of the phylogenetic tree and the species demarcation criteria of the International Committee on Taxonomy of Viruses (ICTV) for the genus , MRaTV1 is considered a new member of the genus .
杨梅（Myrica rubra）是原产于东亚、广泛种植于中国中南部的一种具有重要经济价值的果树。然而，有关感染杨梅的病毒的研究还很缺乏。在本研究中，我们采用宏转录组学方法，鉴定了 2022 年在中国福建省福州市采集的出现黄化和不规则边缘症状的杨梅叶片中的病毒。结果鉴定出一个新的 属病毒，并暂定名为“杨梅伴番茄丛矮病毒 1”（MRaTV1）。通过重叠反转录聚合酶链反应（RT-PCR）和快速扩增 cDNA 末端（RACE）确定了 MRaTV1 的基因组测序。MRaTV1 编码的两个推定蛋白与 属内 科的 Panax notoginseng virus A（PNVA）的外壳蛋白（CP）和 RNA 依赖性 RNA 聚合酶（RdRP）具有最高的氨基酸（aa）序列同一性，分别为 49.7%和 61.7%。根据系统发育树和国际病毒分类委员会（ICTV）对 属的种划分标准，MRaTV1 被认为是该属的一个新成员。
