Huwanixi Aishuake, Peng Zhao, Li Shenglan, Zhou Yutian, Zhao Sixian, Wan Cuihong
School of Life Sciences and Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University, Wuhan, Hubei 430079, People's Republic of China.
School of Life Sciences and Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University, Wuhan, Hubei 430079, People's Republic of China.
J Proteomics. 2024 Apr 15;297:105130. doi: 10.1016/j.jprot.2024.105130. Epub 2024 Feb 23.
Seed germination, a key initial event in the plant life cycle, directly affects cotton yield and quality. Gossypium barbadense and Gossypium hirsutum gradually evolved through polyploidization, resulting in different characteristics, and this interspecific variation lacks genetic and molecular explanation. This work aimed to compare the proteomes between G. barbadense and G. hirsutum during seed germination. Here, we identified 2740 proteins for G. barbadense and 3758 for G. hirsutum. In the initial state, proteins in two cotton involved similar bioprocess, such as sugar metabolism, DNA repairing, and ABA signaling pathway. However, in the post-germination stage, G. hirsutum expressed more protein related to redox homeostasis, peroxidase activity, and pathogen interactions. Analyzing the different expression patterns of 915 single-copy orthogroups between the two kinds of cotton indicated that most of the differentially expressed proteins in G. barbadense were related to carbon metabolism. In contrast, most proteins in G. hirsutum were associated with stress response. Besides that, by proteogenomic analysis, we found 349 putative non-canonical peptides, which may be involved in plant development. These results will help to understand the different characteristics of these two kinds of cotton, such as fiber quality, yield, and adaptability. SIGNIFICANCE STATEMENT: Cotton is the predominant natural fiber crop worldwide; Gossypium barbadense and Gossypium hirsutum have evolved through polyploidization to produce differing traits. However, given their specific features, the divergence of mechanisms underlying seed germination between G. hirsutum and G. barbadense has not been discussed. Here, we explore what protein contributes to interspecific differences between G. barbadense and G. hirsutum during the seed germination period. This study helps to elucidate the evolution and domestication history of cotton polyploids and may allow breeders to understand their domestication history better and improve fiber quality and adaptability.
种子萌发是植物生命周期中的一个关键起始事件，直接影响棉花的产量和品质。陆地棉和海岛棉通过多倍体化逐渐进化，产生了不同的特性，而这种种间变异缺乏遗传和分子层面的解释。这项工作旨在比较海岛棉和陆地棉种子萌发过程中的蛋白质组。在此，我们鉴定出海岛棉有2740种蛋白质，陆地棉有3758种蛋白质。在初始状态下，两种棉花中的蛋白质参与相似的生物过程，如糖代谢、DNA修复和脱落酸信号通路。然而，在萌发后期，陆地棉表达了更多与氧化还原稳态、过氧化物酶活性和病原体相互作用相关的蛋白质。对两种棉花之间915个单拷贝直系同源组的差异表达模式进行分析表明，海岛棉中大多数差异表达的蛋白质与碳代谢有关。相比之下，陆地棉中的大多数蛋白质与应激反应相关。除此之外，通过蛋白质基因组分析，我们发现了349个推定的非经典肽，它们可能参与植物发育。这些结果将有助于理解这两种棉花在纤维品质、产量和适应性等方面的不同特性。意义声明：棉花是全球主要的天然纤维作物；海岛棉和陆地棉通过多倍体化进化产生了不同的性状。然而，鉴于它们的具体特征，陆地棉和海岛棉种子萌发潜在机制的差异尚未得到讨论。在此，我们探究在种子萌发期间哪些蛋白质导致了海岛棉和陆地棉之间的种间差异。这项研究有助于阐明棉花多倍体的进化和驯化历史，并可能使育种者更好地了解它们的驯化历史，从而改善纤维品质和适应性。
