Laboratory of Integrative Neuroendocrinology, Department of Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B4, Canada; Fisheries Department, Faculty of Natural Resources, University of Guilan, Sowmeh Sara, Guilan, Iran.
Laboratory of Integrative Neuroendocrinology, Department of Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B4, Canada.
Comp Biochem Physiol A Mol Integr Physiol. 2024 Jun;292:111609. doi: 10.1016/j.cbpa.2024.111609. Epub 2024 Feb 23.
Kisspeptin is a multifunctional neurohormone, primarily involved in the regulation of reproduction. We tested whether peripheral administration of kisspeptin10 (KP-10) via intraperitoneal injection or slow release affects reproductive hormones and metabolites in Sterlet sturgeon (Acipenser ruthenus). Plasma and mucus 17β-estradiol (E), and testosterone (T), plasma and follicular vitellogenin (VTG) and calcium (Ca) as well as glucose and lipids were determined. Mature Sterlet sturgeon were grouped into six groups: saline i.p injection (control), human kisspeptin (hKP-10) i.p injection; acipenser kisspeptin (aKP-10) i.p injection; hKP-10 (slow release); aKP-10 (slow-release) and no treatment control. No effect for KP-10 on sturgeon body weight was found after 4 weeks of treatment. Multivariate analysis revealed a significant disparity in plasma E levels. It was significantly different between groups (time, P = 0.0022). E in epithelia mucosa showed significant difference between and within groups in the acute group (time, P = 0.0252; treatment, P = 0.0423; time × treatment, P = 0.0429). T levels were unaffected by treatments (P > 0.05). The presence of synthetic aKP-10 led to an elevation in oocyte and plasma VTG levels (P < 0.05). Prolonged exposure to this peptide resulted in an increase in plasma calcium levels. Simultaneously, there was an augmentation in the number of mature follicles. Regardless of the duration of exposure, aKP-10 significantly elevated plasma glucose levels in Sterlet (P < 0.0). Additionally, KP-10 led to an increase in plasma lipids and cholesterol in Sterlet. Overall, our data support an involvement for KP-10 in the regulation of gonadal steroid hormones, oocyte maturation and metabolite levels in sturgeon, suggesting a positive role for this peptide in the reproductive physiology of this species.
Kisspeptin 是一种多功能神经激素，主要参与生殖调节。我们测试了通过腹腔注射或缓释方式给予 kisspeptin10（KP-10）是否会影响史氏鲟（Acipenser ruthenus）的生殖激素和代谢物。测定了血浆和黏液中的 17β-雌二醇（E）和睾酮（T）、血浆和卵泡卵黄蛋白原（VTG）和钙（Ca）以及葡萄糖和脂质。将成熟的史氏鲟分为六组：生理盐水腹腔注射（对照）、人 kisspeptin（hKP-10）腹腔注射；鲟鱼 kisspeptin（aKP-10）腹腔注射；hKP-10（缓释）；aKP-10（缓释）和无处理对照。经过 4 周的治疗，KP-10 对史氏鲟体重没有影响。多变量分析显示，血浆 E 水平存在显著差异。组间差异有统计学意义（时间，P=0.0022）。急性组上皮黏膜中的 E 在组间和组内均有显著差异（时间，P=0.0252；处理，P=0.0423；时间×处理，P=0.0429）。处理对 T 水平没有影响（P>0.05）。合成 aKP-10 的存在导致卵母细胞和血浆 VTG 水平升高（P<0.05）。长时间暴露于这种肽会导致血浆钙水平升高。同时，成熟卵泡数量增加。无论暴露时间长短，aKP-10 都会显著升高史氏鲟的血浆葡萄糖水平（P<0.0）。此外，KP-10 导致史氏鲟血浆脂质和胆固醇升高。总的来说，我们的数据支持 KP-10 参与调控鲟鱼的性腺类固醇激素、卵母细胞成熟和代谢物水平，表明这种肽在该物种的生殖生理中发挥积极作用。
