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超感染的大肠杆菌recA(P1)溶原菌中的原噬菌体替换和原噬菌体丢失

Prophage substitution and prophage loss from superinfected Escherichia coli recA(P1) lysogens.

作者信息

Meurs E, D'Ari R

出版信息

J Virol. 1979 Aug;31(2):277-80. doi: 10.1128/JVI.31.2.277-280.1979.

Abstract

It is shown that the plasmid prophage P1 can be displaced by a superinfecting P1 phage in Escherichia coli recA(P1) lysogens. Six widely separated phage markers were used to distinguish between residual recombination and total substitution. It is further shown that superinfection of recA lysogens can lead to loss of both phage (curing). These two phenomena, previously reported in Rec+ strains, are thus independent of host recombination and may result from perturbations of some function involved in plasmid maintenance.

摘要

结果表明,在大肠杆菌recA(P1)溶原菌中,质粒原噬菌体P1可被超感染的P1噬菌体取代。使用六个相距较远的噬菌体标记来区分残留重组和完全取代。进一步表明,recA溶原菌的超感染可导致两种噬菌体的丢失(治愈)。这两种先前在Rec+菌株中报道过的现象,因此与宿主重组无关,可能是由质粒维持中某些功能的扰动引起的。

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