Lei Zhubing, Pang Xinpei, Li Li, Mei Qian, Dong Wenfei
School of Biomedical Engineering (Suzhou), University of Science and Technology of China, Suzhou, Jiangsu 215163, P. R. China.
Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, Jiangsu 215163, P. R. China.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2024 Feb 25;41(1):152-159. doi: 10.7507/1001-5515.202304061.
A miniaturized, low-cost high-intensity focused ultrasound device is developed for the problems of cross-contamination and uneven sample fragmentation in conventional ultrasound devices. This device generates ultrasonic waves through a concave spherical self-focusing piezoelectric ceramic piece, and creates a cavitation effect in the focusing area to achieve sample fragmentation. The feasibility of the device is demonstrated by physical simulation, then a driving circuit with adjustable power is designed and manufactured to generate 0 ~ 22.4 W acoustic power, and finally paraffin-embedded sample dewaxing experiments are performed to verify the validation of the device. The experimental results show that the dewaxing efficiency and safety of the high-intensity focused ultrasound device is significantly better than those of traditional chemical methods, and this device is comparable with commercial ultrasonic instruments. In summary, the high-intensity focused ultrasound device is expected to be applied in automated nucleic acid extraction and purification equipment and has a broad application prospect in the field of sample pre-processing.
针对传统超声设备存在的交叉污染和样本破碎不均等问题，研发了一种小型化、低成本的高强度聚焦超声设备。该设备通过凹球面自聚焦压电陶瓷片产生超声波，并在聚焦区域产生空化效应以实现样本破碎。通过物理模拟证明了该设备的可行性，然后设计并制造了功率可调的驱动电路，以产生0~22.4W的声功率，最后进行石蜡包埋样本脱蜡实验以验证该设备的有效性。实验结果表明，高强度聚焦超声设备的脱蜡效率和安全性明显优于传统化学方法，且该设备与商用超声仪器相当。综上所述，高强度聚焦超声设备有望应用于自动化核酸提取和纯化设备，在样本预处理领域具有广阔的应用前景。
