对从正常受试者和患有各种疾病的患者中获得的人甲状腺球蛋白寡糖的比较研究。
Comparative study of the oligosaccharides of human thyroglobulins obtained from normal subjects and patients with various diseases.
作者信息
Hotta T, Ishii I, Ishihara H, Tejima S, Tarutani O, Takahashi N
出版信息
J Appl Biochem. 1985 Apr;7(2):98-103.
Asparagine-linked oligosaccharides were released quantitatively by N-oligosaccharide glycopeptidase (almond) digestion from human thyroglobulins prepared from thyroid glands of normal subjects and patients with several pathological conditions. The pyridylamino derivatives of the oligosaccharides were prepared and analyzed by high-performance liquid chromatography. The content of high-mannose-type oligosaccharides was comparable to that of the complex type in normal thyroglobulins. Man9GlcNAc2 was the predominant component in the high-mannose-type region, while biantennary oligosaccharides with fucose were the major components in the complex-type region. High-mannose-type oligosaccharides were markedly decreased in thyroglobulins prepared from patients with various disorders, such as Basedow's disease, papillary carcinoma, and adenomatous goiter, whereas they were appreciably increased in thyroglobulin from diffuse goiter.
通过N-寡糖糖肽酶（杏仁）消化，从正常受试者以及患有多种病理疾病患者的甲状腺中制备的人甲状腺球蛋白中定量释放出天冬酰胺连接的寡糖。制备寡糖的吡啶基氨基衍生物，并通过高效液相色谱法进行分析。正常甲状腺球蛋白中高甘露糖型寡糖的含量与复合型相当。Man9GlcNAc2是高甘露糖型区域的主要成分，而带有岩藻糖的双天线寡糖是复合型区域的主要成分。在由各种疾病患者制备的甲状腺球蛋白中，如突眼性甲状腺肿、乳头状癌和腺瘤性甲状腺肿，高甘露糖型寡糖明显减少，而在弥漫性甲状腺肿患者的甲状腺球蛋白中则明显增加。
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